Tissue ablation with irreversible electroporation

• US 8,048,067 B2
• Filed: 12/21/2004
• Issued: 11/01/2011
• Est. Priority Date: 12/24/2003
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Rubinsky, Boris, Davalos, Rafael
• Primary Examiner(s): Peffley; Michael
• Assistant Examiner(s): Hupczey, Jr.; Ronald
• Application Number:
  US10/571,162
• Publication Number:
  US 20070043345A1
• Time in Patent Office: 2,506 Days
• Field of Search: 606 32- 50
• US Class Current: 606/32
• CPC Class Codes: A61B 18/12 by passing a current through the tissue to be heated, e.g. high-frequency current
• CPC Class Codes: A61B 18/1233 with circuits for assuring patient safety
• CPC Class Codes: A61B 18/1477 Needle-like probes
• CPC Class Codes: A61B 2018/00577 Ablation
• CPC Class Codes: A61B 2018/00613 Irreversible electroporation reversible electroporation A61N1/327
• CPC Class Codes: A61B 2018/00714 Temperature
• CPC Class Codes: A61B 2018/00761 Duration
• CPC Class Codes: A61B 2018/00827 Current
• CPC Class Codes: A61B 2018/1425 Needle
• CPC Class Codes: A61N 1/0412 Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
• CPC Class Codes: A61N 1/0472 Structure-related aspects
• CPC Class Codes: A61N 1/05 for implantation or insertion into the body, e.g. heart electrode A61N1/06 takes precedence
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation

Abstract

A new method for the ablation of undesirable tissue such as cells of a cancerous or non-cancerous tumor is disclosed. It involves the placement of electrodes into or near the vicinity of the undesirable tissue through the application of electrical pulses causing irreversible electroporation of the cells throughout the entire area of the undesirable tissue. The electric pulses irreversibly permeate the cell membranes, thereby invoking cell death. The irreversibly permeabilized cells are left in situ and are removed by the body immune system. The amount of tissue ablation achievable through the use of irreversible electroporation without inducing thermal damage is considerable.

Claims

1. A method of ablating tissue cells by non-thermal irreversible electroporation, comprising the steps of:

(a) identifying tissue to be ablated in a living mammal;

(b) placing a first electrode and a second electrode such that the identified tissue is positioned between the first and second electrodes; and

(c) applying electrical pulses between the first and second electrodes to the identified tissue in the living mammal, in an amount which has been predetermined to be sufficient to induce irreversible electroporation of cells of the identified tissue, but which has been predetermined to be insufficient to induce thermal damage to the identified tissue such that the identified tissue cells are killed by irreversible electroporation.

2. The method of claim 1, wherein the electrical pulses are applied for a duration in a range of from about 5 microseconds to about 62 seconds from about 2 to about 15 pulses are applied, and the pulses produce a voltage gradient in a range of from about 50 volt/cm to about 8000 volt/cm.

3. The method of claim 1, wherein the electrical pulses are applied for a period of about 100 microseconds, ±

about 10 microseconds;

further wherein from about 2 to about 15 pulses are applied; and

still further wherein the pulses produce a voltage gradient in a range of from about 50 volt/cm to about 8000 volt/cm.

4. The method of claim 1, wherein about eight pulses of about 100 microseconds each in duration are applied.

5. The method of claim 1, wherein the first electrode is placed at about 5 mm to 10 cm from the second electrode.

6. The method of claim 1, wherein the first electrode and second electrode are circular in shape.

7. The method of claim 1, wherein the electrical pulses are applied between the first and second electrodes in an amount which has been predetermined to maintain the temperature of the target tissue at 50°

C. or less.

8. The method of claim 1, further comprising:

continuously detecting the electrical impedance of the identified tissue to determine an occurrence of irreversible electroporation of the identified tissue.

9. The method of claim 8, wherein the step of continuously detecting includes detecting the electrical impedance of the identified tissue to determine a point where, on average, the cells of the identified tissue have undergone irreversible electroporation.

10. A method of ablating tissue cells by non-thermal irreversible electroporation, comprising the steps of:

identifying a tissue zone to be treated in a living mammal;

placing first and second electrodes such that the identified tissue zone is positioned between the first and second electrodes;

applying a predetermined electric field to the identified tissue zone in an amount which is sufficient to perform irreversible electroporation of cells in the identified tissue zone, but which is insufficient to induce thermal damage to the cells in the identified tissue zone to kill substantially all of the cells in the identified tissue zone by irreversible electroporation.

11. The method of claim 10, wherein the irreversible electroporation results in a fatal disruption of the cells'"'"' normal controlled flow of material across a membrane of the cell.

12. The method of claim 10, further comprising:

continuously detecting the electrical impedance of the identified tissue zone to determine an occurrence of irreversible electroporation of the identified tissue zone.

13. The method of claim 12, wherein the step of continuously detecting includes detecting the electrical impedance of the identified tissue zone to determine a point where, on average, the cells of the identified tissue zone have undergone irreversible electroporation.