Electrospun blends of natural and synthetic polymer fibers as tissue engineering scaffolds

• US 8,048,446 B2
• Filed: 05/10/2006
• Issued: 11/01/2011
• Est. Priority Date: 05/10/2005
• Status: Expired due to Fees

• Current Assignee: Drexel University
• Original Assignee: Drexel University
• Inventors: Ko, Frank, Mondrinos, Mark, Li, Mengyan, Lelkes, Peter I.
• Primary Examiner(s): Kosson; Rosanne
• Application Number:
  US11/431,484
• Publication Number:
  US 20060263417A1
• Time in Patent Office: 2,001 Days
• Field of Search: 424/443, 424/422, 530/353, 530/354, 528/272, 514/801
• US Class Current: 424/443
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: D01D 5/0038 the fibre formed by solvent evaporation, i.e. dry electro-spinning
• CPC Class Codes: D01F 6/92 of polyesters
• CPC Class Codes: Y10T 442/2525 Coating or impregnation functions biologically [e.g., insect repellent, antiseptic, insecticide, bactericide, etc.]

Abstract

Non-woven fibrous scaffolds made by electrospinning from the synthetic biodegradable polymer such as, for example, poly(lactic-co-glycolic acid) (PLGA) and natural proteins, such as, for example, gelatin (denatured collagen) and elastin and a method of making thereof.

Claims

1. A fiber comprising an electrospun blend of at least one synthetic polymer and at least two natural polymers, wherein none of the polymers is crosslinked, wherein said synthetic polymer is poly (lactide-co-glycolide) (PLGA) and wherein the at least two natural polymers are elastin and gelatin, further wherein said electrospun blend comprises said synthetic polymer, gelatin and elastin at a ratio of 30:

8;

40.

2. A scaffold made from the fiber of claim 1.

3. The scaffold of claim 2, further comprising a cell.

4. The scaffold of claim 2, further comprising a pharmaceutical agent.

5. The fiber of claim 1, further comprising a pharmaceutical agent.

6. A method of making the fiber of claim 1, the method comprising the steps of:

(a) providing a blend of at least one synthetic polymer and at least two natural polymers, wherein none of the polymers is crosslinked, wherein said synthetic polymer is poly (lactide-co-glycolide) (PLGA) and wherein the at least two natural polymers are elastin and gelatin, further wherein said electrospun blend comprises said synthetic polymer, gelatin and elastin at a ratio of 30;

8;

40; and

(b) electrospinning the blend to form the fiber.

7. A fiber comprising an electrospun blend of at least one synthetic polymer and at least two natural polymers, wherein none of the polymers is crosslinked, wherein said synthetic polymer is poly (lactide-co-glycolide) (PLGA) and wherein the at least two natural polymers are elastin and gelatin, further wherein said electrospun blend comprises said synthetic polymer, gelatin and elastin at a ratio of 20:

16;

40.

8. A scaffold made from the fiber of claim 7.

9. The scaffold of claim 8, further comprising a cell.

10. The scaffold of claim 8, further comprising a pharmaceutical agent.

11. The fiber of claim 7, further comprising a pharmaceutical agent.

12. A method of making the fiber of claim 7, the method comprising the steps of:

(a) providing a blend of at least one synthetic polymer and at least two natural polymers, wherein none of the polymers is crosslinked, wherein said synthetic polymer is poly (lactide-co-glycolide) (PLGA) and wherein the at least two natural polymers are elastin and gelatin, further wherein said electrospun blend comprises said synthetic polymer, gelatin and elastin at a ratio of 20;

16;

40; and

(b) electrospinning the blend to form the fiber.