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Methods for nucleic acid amplification on a printed circuit board

  • US 8,048,628 B2
  • Filed: 05/24/2007
  • Issued: 11/01/2011
  • Est. Priority Date: 09/24/2002
  • Status: Expired due to Fees
First Claim
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1. A method of amplifying DNA comprising:

  • (a) providing a sample solution comprising DNA on a printed circuit board substrate comprising an array of electrodes for transporting droplets on the substrate, and a dielectric coating atop the electrodes, wherein the printed circuit board substrate is provided as part of a sandwich structure, the sandwich structure further comprising;

    (i) a top plate parallel to the printed circuit board substrate and forming a gap between the top plate and the printed circuit board substrate;

    (ii) a seal between the top plate and the printed circuit board substrate; and

    (iii) oil in the gap; and

    (b) amplifying the DNA on the printed circuit board substrate, wherein the amplifying is achieved by thermocycling wherein the amplifying is accomplished in a droplet in the oil in the gap.

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