Heparanase specific molecular probes and their use in research and medical applications

• US 8,048,993 B2
• Filed: 01/04/2008
• Issued: 11/01/2011
• Est. Priority Date: 09/02/1997
• Status: Expired due to Fees

• Current Assignee: Hadasit Medical Research Services and Development Limited, Insight Biopharmaceuticals Limited
• Original Assignee: Hadasit Medical Research Services & Development Ltd (Hadassah, The Women's Zionist Organization of America, Inc.), Insight Biopharmaceuticals Limited
• Inventors: Friedmann, Yael, Peretz, Tuvia, Vlodavsky, Israel, Pecker, Iris
• Primary Examiner(s): Ewoldt; Gerald R
• Assistant Examiner(s): Dibrino; Marianne
• Application Number:
  US12/003,999
• Publication Number:
  US 20090099341A1
• Time in Patent Office: 1,397 Days
• Field of Search: None
• US Class Current: 530/387.1
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61M 15/009 using medicine packages with incorporated spraying means, e.g. aerosol cans pocket atomiser of the injector type A61M11/08
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C07K 16/40 against enzymes
• CPC Class Codes: C12N 9/2402 hydrolysing O- and S- glycosyl compounds (3.2.1)
• CPC Class Codes: C12Y 302/01166 Heparanase (3.2.1.166)
• CPC Class Codes: G01N 2333/988 Lyases (4.), e.g. aldolases, heparinase, enolases, fumarase
• CPC Class Codes: G01N 2400/40 Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
• CPC Class Codes: G01N 33/573 for enzymes or isoenzymes

Abstract

According to the present invention there is provided an antibody that specifically binds an epitope of a heparanase protein, the heparanase protein including an amino acid sequence as set forth in SEQ ID NO:2, provided that phenylalanine replaces tyrosine at position 246. The present invention also provides an antibody elicited by an epitope of a heparanase protein, the heparanase protein including an amino acid sequence as set forth in SEQ ID NO:2, provided that phenylalanine replaces tyrosine at position 246.

Claims

1. An antibody specifically binding an epitope of a heparanase protein, said heparanase protein comprising the amino acid sequence as set forth in SEQ ID NO:

2, provided that phenylalanine replaces tyrosine at position 246.

2. The antibody of claim 1, wherein said heparanase protein consists of said amino acid sequence.

3. The antibody of claim 1, wherein said heparanase protein is recombinant.

4. The antibody of claim 1, wherein elicitation of the antibody is through in vivo or in vitro techniques, said antibody having been prepared by a process comprising the steps of:

(a) exposing cells capable of producing antibodies to said at least one epitope of said heparanase protein and thereby generating antibody producing cells;

(b) fusing said antibody producing cells with myeloma cells and thereby generating a plurality of hybridoma cells each producing monoclonal antibodies; and

(c) screening said plurality of monoclonal antibodies to identify a monoclonal antibody which specifically binds heparanase.

5. The antibody of claim 1, wherein the antibody is selected from the group consisting of a polyclonal antibody and a monoclonal antibody.

6. The antibody of claim 5, wherein said polyclonal antibody is selected from the group consisting of a crude polyclonal antibody and an affinity purified polyclonal antibody.

7. An antibody elicited by an epitope of a heparanase protein, said heparanase protein comprising the amino acid sequence as set forth in SEQ ID NO:

2, provided that phenylalanine replaces tyrosine at position 246.

8. The antibody of claim 7, wherein said heparanase protein consists of said amino acid sequence.

9. The antibody of claim 7, wherein said heparanase protein is recombinant.

10. The antibody of claim 7, wherein elicitation of the antibody is through in vivo or in vitro techniques, said antibody having been prepared by a process comprising the steps of:

(a) exposing cells capable of producing antibodies to said at least one epitope of said heparanase protein and thereby generating antibody producing cells;

(b) fusing said antibody producing cells with myeloma cells and thereby generating a plurality of hybridoma cells each producing monoclonal antibodies; and

(c) screening said plurality of monoclonal antibodies to identify a monoclonal antibody which specifically binds heparanase.

11. The antibody of claim 7, wherein the antibody is selected from the group consisting of a polyclonal antibody and a monoclonal antibody.

12. The antibody of claim 11, wherein said polyclonal antibody is selected from the group consisting of a crude polyclonal antibody and an affinity purified polyclonal antibody.