Genes involved in plant fibre development

• US 8,049,069 B2
• Filed: 03/31/2005
• Issued: 11/01/2011
• Est. Priority Date: 03/31/2004
• Status: Active Grant

• Current Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Original Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Inventors: Dennis, Elizabeth Salisbury, Llewellyn, Danny James, Machado, Adriane Cristine, Wu, Yingru
• Primary Examiner(s): Baum; Stuart F.
• Application Number:
  US10/594,785
• Publication Number:
  US 20080196120A1
• Time in Patent Office: 2,406 Days
• Field of Search: None
• US Class Current: 800/298
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8242 with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits

Abstract

The present invention provides polypeptides, and polynucleotides encoding therefore, involved in the regulation of fibre initiation and/or elongation in fibre producing plants. In particular, the present invention provides methods of altering fibre initiation in cotton making use of transcription factors, regulatory proteins or cell cycle proteins produced at or around anthesis. The invention also relates to the use of these as markers of fibre production in plants including cotton.

Claims

1. A method of increasing fibre initiation and/or elongation in a fibre producing plant comprising introducing into the plant a nucleotide sequence encoding a polypeptide comprising consecutive amino acids whose sequence is identical to the amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO:

38, such that the production of the polypeptide is increased when compared to a wild-type fiber producing plant, wherein the nucleotide sequence had been previously isolated.

3. The method of claim 1 or 2, wherein the polypeptide comprises consecutive amino acids whose sequence is set forth in SEQ ID NO:

12.

5. The method of claim 1 or 2, wherein the plant is a species of the Genus Gossypium.

2. A method of reducing fibre initiation and/or elongation in a fibre producing plant comprising introducing into the plant an antisense polynucleotide or a polynucleotide that produces a dsRNA molecule such that the production of a polypeptide comprising consecutive amino acids whose sequence is identical to the amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO:

38 is reduced when compared to a wild-type fiber producing plant, wherein the polynucleotide sequence that produces the dsRNA had been previously isolated.

4. The method of claim 2, wherein the dsRNA molecule is double-stranded over at least 19 basepairs whose sequence corresponds to a consecutive sequence set forth in SEQ ID NO:

38, or to a consecutive sequence which is identical to the sequence set forth in SEQ ID NO;

38.

6. A substantially purified and/or recombinant polypeptide comprising consecutive amino acids whose sequence is set forth in SEQ ID NO:

12.

7. An isolated and/or exogenous polynucleotide comprising a polynucleotide selected from the group consisting of:

i) a polynucleotide comprising consecutive nucleotides whose nucleotide sequence is set forth in SEQ ID NO;

38; and

ii) a polynucleotide which encodes a polypeptide comprising consecutive amino acids whose sequence is identical to the amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO;

38.

8. A vector comprising the polynucleotide of claim 7.

9. A plant or bacterial cell comprising the vector of claim 8.

10. A transgenic plant which comprises the exogenous polynucleotide of claim 7.

11. The transgenic plant of claim 10, which when compared to an isogenic non-transgenic plant, produces an increased level of a polypeptide comprising consecutive amino acids whose sequence is identical to the amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO:

38.

12. The transgenic plant of claim 11, wherein the polypeptide comprises consecutive amino acids whose amino acid sequence is set forth in SEQ ID NO:

12.

13. A transgenic seed of the plant of claim 10 comprising said polynucleotide.

14. A process for producing fibre comprising obtaining the transgenic plant of claim 10 so as to thereby produce the fibre.