Culture dish with lid

• US 8,053,230 B2
• Filed: 08/28/2007
• Issued: 11/08/2011
• Est. Priority Date: 09/07/2006
• Status: Active Grant

• Current Assignee: Nagle Nunc International, Nalge NUNC International Corporation (Perspecta, Inc.)
• Original Assignee: Nalge NUNC International Corporation (Perspecta, Inc.)
• Inventors: Whittlinger, Keith Owen
• Primary Examiner(s): Beisner; William H
• Assistant Examiner(s): HOBBS, MICHAEL L
• Application Number:
  US11/846,193
• Publication Number:
  US 20080064090A1
• Time in Patent Office: 1,533 Days
• Field of Search: 4353051-3054, 422/503, 422/536, 422/552, 422/559, 422/569
• US Class Current: 435/305.2
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 9/56 Means for indicating position of a recipient or sample in an array
• CPC Class Codes: C12M 23/10 Petri dish crystallising dishes B01L3/06
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 27/20 Baffles; Ribs; Ribbons; Auger vanes

Abstract

A device for culturing cells or tissue includes a culture dish with a bottom wall and at least one sidewall to define an interior cavity, at least one channel formed on the bottom wall to minimize the effect of fluid movement on biologics in the dish and, optionally, at least one barrier wall for partitioning the interior cavity into a plurality of compartments. The barrier wall maintains a biologic in one compartment separate from a biologic in another compartment. The barrier wall may also provide fluid communication between compartments.

Claims

1. A device for culturing a biologic in a culture medium, the device comprising:

a culture dish including a bottom wall and at least one sidewall projecting therefrom to define an interior cavity, the bottom wall being generally planar and configured to support the biologic thereon;

a pair of spaced apart ridges formed side-by-side in the bottom wall and extending in a common direction to define an elongated channel therebetween, with each of the pair of ridges projecting upwardly from the bottom wall along a respective entire length thereof and having a height that is sufficient to baffle the motion of the culture medium so as to reduce fluid disturbances of the biologic during use;

a first compartment located on one side of the pair of ridges;

a second compartment located on the other side of the pair of ridges; and

at least one barrier wall within at least one of the first or second compartments for partitioning the at least one of the first or second compartments into first and second interior spaces for receiving at least one of a first and a second biologic respectively therein, the at least one barrier wall having a height configured to maintain the first biologic in the first interior space separate from the second biologic in the second interior space,

wherein the height of the ridges is less than the height of the at least one barrier wall.

2. The device of claim 1, wherein the at least one barrier wall includes at least one opening for providing fluid communication between the first interior space and the second interior space.

3. The device of claim 1, wherein the at least one barrier wall includes a plurality of discrete barrier blocks including a gap between adjacent blocks to provide fluid communication.

4. The device of claim 1, further comprising:

a lid adapted to be removably coupled to the culture dish to at least partially cover the interior cavity, the lid including a top wall and at least one sidewall projecting therefrom.

5. The device of claim 4, further comprising:

a least one spacer on one of the culture dish or the lid for providing at least a portion of a fluid flow path from outside the device to the interior cavity of the culture dish.

6. The device of claim 4, further comprising:

a first projection on the culture dish and a second projection on the lid such that when multiple devices are in a stacked configuration, the first projection on one device cooperates with the second projection on an adjacent device to limit movement of the one device relative to the adjacent device.

7. The device of claim 4, further comprising a first orientation indicator for indicating a specified orientation of the lid relative to the culture dish.

8. The device of claim 7, wherein the first orientation indicator further comprises:

a first orientation indicia on one of the culture dish or the lid; and

a second orientation indicia on the other of the culture dish or the lid,

wherein aligning the first orientation indicia with the second orientation indicia provides the specified orientation of the lid relative to the culture dish.

9. The device of claim 7, wherein the orientation indicator includes at least one of a number, letter, symbol, or combinations thereof.

10. The device of claim 1, further comprising a plurality of indicia for identifying a respective interior space in the culture dish.

11. The device of claim 1, wherein the elongated channel extends across substantially the entire dimension of the bottom wall.

12. The device of claim 1, comprising a plurality of pairs of spaced apart ridges defining multiple channels formed in the bottom wall.

13. The device of claim 1, wherein the culture dish has a width of about 8.75 inches and a length of about 8.75 inches.

14. The device of claim 13, wherein the culture dish has a height of about 1.25 inches.

15. The device of claim 13, wherein the at least one barrier wall has a height of about 0.43 inches.

16. The device of claim 13, wherein each of the pair of ridges have a height of about 0.07 inches.