Methods and systems for image processing of microfluidic devices
First Claim
1. A method of processing data associated with fluorescent emissions from a microfluidic device, the method comprising:
- performing an auto-focus process associated with a first image of the microfluidic device;
performing an auto-exposure process associated with the first image of the microfluidic device;
capturing a plurality of images of the microfluidic device, wherein the plurality of images are associated with a plurality of thermal cycles;
performing image analysis of the plurality of captured images to determine a series of optical intensities; and
performing data analysis of the series of optical intensities to provide a series of changes in threshold values.
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Abstract
A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.
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Citations
25 Claims
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1. A method of processing data associated with fluorescent emissions from a microfluidic device, the method comprising:
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performing an auto-focus process associated with a first image of the microfluidic device; performing an auto-exposure process associated with the first image of the microfluidic device; capturing a plurality of images of the microfluidic device, wherein the plurality of images are associated with a plurality of thermal cycles; performing image analysis of the plurality of captured images to determine a series of optical intensities; and performing data analysis of the series of optical intensities to provide a series of changes in threshold values. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of performing an auto-focus process for a microfluidic device including a plurality of chambers, the method comprising:
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acquiring an image stack comprising a plurality of images; determining a focal plane characterized by a predetermined contrast score; determining a plurality of positions associated with the plurality of chambers; computing a contrast score for each of the plurality of images in the image stack; determining an updated focal plane characterized by a second predetermined contrast score; and storing the updated focal plane in one or more memories. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method of determining a baseline value associated with fluorescent emissions from a microfluidic device, the method comprising:
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computing an initial baseline value; providing a first baseline value having a different value than the initial baseline value; providing a series of emission data points as a function of PCR cycle number; subtracting the initial baseline value from the series of emission data points to provide an initial baseline removed curve; computing an initial quality factor associated with initial baseline removed curve; subtracting the first baseline value from the series of emission data points to provide a first baseline removed curve; computing a first quality factor associated with the first baseline removed curve; determining that the first quality factor is greater than the second quality factor; and determining that the baseline value is the first baseline value. - View Dependent Claims (22, 23, 24, 25)
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Specification