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Methods and systems for image processing of microfluidic devices

  • US 8,055,034 B2
  • Filed: 09/13/2007
  • Issued: 11/08/2011
  • Est. Priority Date: 09/13/2006
  • Status: Expired due to Fees
First Claim
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1. A method of processing data associated with fluorescent emissions from a microfluidic device, the method comprising:

  • performing an auto-focus process associated with a first image of the microfluidic device;

    performing an auto-exposure process associated with the first image of the microfluidic device;

    capturing a plurality of images of the microfluidic device, wherein the plurality of images are associated with a plurality of thermal cycles;

    performing image analysis of the plurality of captured images to determine a series of optical intensities; and

    performing data analysis of the series of optical intensities to provide a series of changes in threshold values.

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