Placental stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells
First Claim
1. A composition comprising human stem or progenitor cells and a population of isolated CD34−
- human placental stem cells, wherein said placental stem cells are obtained from a placenta that has been drained of cord blood and flushed to remove residual blood.
3 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides compositions and methods of using placental stem cells that originate from a postpartum placenta with conventional cord blood compositions or other stem or progenitor cells. The placental stem cells can be used alone or in a mixture with other stem cell populations. In accordance with the present invention, the placental stem cells may be mixed with other stem cell populations, including but not limited to, umbilical cord blood, fetal and neonatal hematopoietic stem cells and progenitor cells, human stem cells and progenitor cells derived from bone marrow. The placental stem cells and the mixed populations of placental stem cells and stem cells have a multitude of uses and applications, including but not limited to, therapeutic uses for transplantation and treatment and prevention of disease, and diagnostic and research uses.
315 Citations
55 Claims
-
1. A composition comprising human stem or progenitor cells and a population of isolated CD34−
- human placental stem cells, wherein said placental stem cells are obtained from a placenta that has been drained of cord blood and flushed to remove residual blood.
- View Dependent Claims (3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 51, 52)
-
2. A composition comprising human umbilical cord blood cells and a population of isolated CD34−
- human placental stem cells, wherein said placental stem cells are obtained from a placenta that has been drained of cord blood and flushed to remove residual blood.
- View Dependent Claims (4, 53, 54)
-
17. A kit comprising (1) CD34−
- placental stem cells, and (2) either stem or progenitor cells, or umbilical cord blood cells;
wherein the cells of (1) are in a first container and the cells of (2) are in a second container. - View Dependent Claims (18, 19, 20, 21)
- placental stem cells, and (2) either stem or progenitor cells, or umbilical cord blood cells;
-
22. A composition comprising human stem or progenitor cells and isolated human CD34−
- placental stem cells, wherein the placental stem cells have been obtained from a placenta that has been drained of cord blood and flushed to remove residual blood, and wherein said placental stem cells comprise a transgene or foreign nucleic acid.
- View Dependent Claims (23, 24)
-
25. A method of making a pharmaceutical composition, comprising combining a plurality of stem or progenitor cells and a population of CD34−
- placental stem cells, wherein said placental stem cells have been obtained from a placenta that has been drained of cord blood and perfused to remove residual blood.
- View Dependent Claims (26, 27, 28, 55)
-
29. A method of differentiating placental stem cells into neural cells, comprising contacting CD34−
- placental stem cells with one or more compounds that cause differentiation of the placental stem cells into neural cells, so that the placental stem cells differentiate into neural cells.
- View Dependent Claims (30)
-
31. A method of differentiating placental stem cells into chondrocytes, comprising contacting CD34−
- placental stem cells with one or more compounds that cause differentiation of the placental stem cells into chondrocytes, so that the placental stem cells differentiate into chondrocytes.
- View Dependent Claims (32)
-
33. A method of differentiating placental stem cells into osteocytes, comprising contacting CD34−
- placental stem cells with one or more compounds that cause differentiation of the placental stem cells into osteocytes, so that the placental stem cells differentiate into osteocytes.
- View Dependent Claims (34)
-
35. A method of differentiating placental stem cells into hepatocytes, comprising contacting CD34−
- placental stem cells with one or more compounds that cause differentiation of the placental stem cells into hepatocytes, so that the placental stem cells differentiate into hepatocytes.
- View Dependent Claims (36)
-
37. A method of differentiating placental stem cells into pancreatic cells, comprising contacting CD34−
- placental stem cells with one or more compounds that cause differentiation of the placental stem cell into pancreatic cells, so that the placental stem cells differentiate into pancreatic cells.
- View Dependent Claims (38)
-
39. A method of differentiating placental stem cells into cardiac cells, comprising contacting CD34−
- placental stem cell with one or more compounds that cause differentiation of the placental stem cells into cardiac cells, so that the placental stem cells differentiate into cardiac cells.
- View Dependent Claims (40)
-
41. A method of treating an individual in need of hematopoietic reconstitution, comprising administering to said individual a population of placental stem cells and umbilical cord blood cells, wherein said population of placental stem cells is SH2+, SH3+, SH4+, and wherein said population of placental stem cells is additionally either OCT-4+, or SSEA3−
- and SSEA4−
, wherein OCT-4 is octamer binding protein 4. - View Dependent Claims (43, 44, 45, 46, 47, 48, 49, 50)
- and SSEA4−
-
42. A method of treating an individual in need of hematopoietic reconstitution, comprising administering to said individual a population of placental stem cells and umbilical cord blood cells, wherein said population of placental stem cells is CD34−
- , SSEA3−
, SSEA4−
, and OCT-4+.
- , SSEA3−
Specification