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Monocyte activation test better able to detect non-endotoxin pyrogenic contaminants in medical products

  • US 8,058,021 B2
  • Filed: 04/26/2010
  • Issued: 11/15/2011
  • Est. Priority Date: 12/22/2005
  • Status: Active Grant
First Claim
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1. A diagnostic kit comprising:

  • a. a first assay system which includes at least one microtiter well shaped such that a monocyte-containing reagent is concentrated to provide greater cell to cell contact as compared to a flat-bottomed microtiter well, wherein the surface of the microtiter well comprises a polypropylene coating or the entire microtiter well is composed of polypropyleneb. a second assay system which comprises at least one surface treated with an antibody to a cytokine or endogenous mediator of the inflammatory response;

    c. instructions for incubating in the first assay system a monocyte-containing reagent and a sample to be tested, transferring the contents of the first assay system to the second assay system, and assaying the second system for the presence of cytokine or endogenous mediator bound to the antibody.

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