Oct3/4, Klf4, c-Myc and Sox2 produce induced pluripotent stem cells
First Claim
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1. A method for preparing an induced pluripotent stem cell by nuclear reprogramming of a somatic cell from a mammalian species, comprising:
- a) introducing into the somatic cell one or more retroviral vectors comprising a gene encoding Oct3/4, a gene encoding Klf4, a gene encoding c-Myc and a gene encoding Sox2 operably linked to a promoter; and
b) culturing the transduced somatic cell on a fibroblast feeder layer or extracellular matrix in a cell media that supports growth of ES cells of the mammalian species, wherein one or more pluripotent cells are obtained.
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Abstract
The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.
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1 Claim
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1. A method for preparing an induced pluripotent stem cell by nuclear reprogramming of a somatic cell from a mammalian species, comprising:
a) introducing into the somatic cell one or more retroviral vectors comprising a gene encoding Oct3/4, a gene encoding Klf4, a gene encoding c-Myc and a gene encoding Sox2 operably linked to a promoter; and
b) culturing the transduced somatic cell on a fibroblast feeder layer or extracellular matrix in a cell media that supports growth of ES cells of the mammalian species, wherein one or more pluripotent cells are obtained.
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Current AssigneeKyoto University
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Original AssigneeKyoto University
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InventorsYamanaka, Shinya, Takahashi, Kazutoshi
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Primary Examiner(s)Crouch, Deborah
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Application NumberUS12/457,356Publication NumberTime in Patent Office889 DaysField of SearchNoneUS Class Current435/377CPC Class CodesA61P 43/00 Drugs for specific purposes...C07K 14/4702 Regulators; Modulating acti...C12N 2501/60 Transcription factorsC12N 2501/602 Sox-2C12N 2501/603 Oct-3/4C12N 2501/604 Klf-4C12N 2510/00 Genetically modified cellsC12N 2740/15043 viral genome or elements th...C12N 5/0696 Artificially induced plurip...
