Multiplex data collection and analysis in bioanalyte detection

• US 8,062,841 B2
• Filed: 06/26/2008
• Issued: 11/22/2011
• Est. Priority Date: 09/01/2005
• Status: Active Grant

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Jones, Richard, Yamakawa, Mineo, Su, Xing, Ma, Qing, Sun, Lei, Zhang, Jingwu, Koo, Tae-Woong
• Primary Examiner(s): Whisenant; Ethan C
• Application Number:
  US12/147,440
• Publication Number:
  US 20090005260A1
• Time in Patent Office: 1,244 Days
• Field of Search: 435/6, 435/7.1, 536/23.1, 536/24.3, 530/350
• US Class Current: 435/6
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a single spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

Claims

1. A method comprising contacting a sample comprising an analyte with a reagent comprising first and second binding partners and first and second reporters, and forming a complex comprising the first binding partner, the first reporter associated with the first binding partner, the analyte, the second binding partner, and the second reporter associated with the second binding partner, further comprising placing a portion of the complex to a detection site, wherein the detection site is a cavity in a MFC or a spot of a microarray, wherein the first reporter or the second reporter comprises a magnetic COIN that comprises a metal particle with at least one Raman active organic compound adsorbed on the metal particle.

2. The method of claim 1, wherein the first reporter is different from the second reporter.

3. The method of claim 2, wherein the complex is formed in solution or on a surface.

4. The method of claim 2, further comprising collecting an electromagnetic spectrum from the portion of the complex in the detection site.

5. The method of claim 4, wherein the electromagnetic spectrum is a Raman spectrum.

6. The method of claim 4, further comprising de-convoluting the electromagnetic spectrum by a computer implemented system to identify the first and second reporters and providing analyte information based on the identity of the first and second reporters.

7. The method of claim 2, wherein the analyte is a bioanalyte containing

information to detect a disease.

8. The method of claim 2, wherein the first reporter or the second reporter comprises a quantum dot, a Raman tag, a fluorescent tag, a COIN, or a magnetic COIN.

9. The method of claim 2, wherein the analyte is located between the first binding partner and the second binding partner.

10. A method comprising contacting a sample comprising an analyte with a first binding partner immobilized on a surface and contacting a reagent comprising second binding partner and a second reporter, and forming a complex comprising the first binding partner, the analyte, the second binding partner, and the second reporter associated with the second binding partner, wherein the surface is a spot enclosed in a MFC or a spot of a microarray, wherein the first reporter or the second reporter comprises a magnetic COIN that comprises a metal particle with at least one Raman active organic compound adsorbed on the metal particle.

11. The method of claim 10, wherein the analyte is located between the first binding partner and the second binding partner.

12. The method of claim 11, wherein the surface is that of a spot enclosed in a MFC.

13. The method of claim 11, further comprising collecting an electromagnetic spectrum from a portion of the complex.

14. The method of claim 13, further comprising de-convoluting the electromagnetic spectrum by a computer implemented system to identify the second reporter and providing analyte information based on the identity of the second reporter.

15. The method of claim 13, wherein the electromagnetic spectrum is a Raman spectrum and the reporter is a COIN or a magnetic COIN.

16. The method of claim 11, further comprising a first reporter associated with the first binding partner.

17. The method of claim 16, wherein the first reporter is different from the second reporter.