Optically readable microplate

• US 8,062,900 B2
• Filed: 05/17/2006
• Issued: 11/22/2011
• Est. Priority Date: 12/30/2005
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Modavis, Robert A.
• Primary Examiner(s): Bullock; In Suk
• Assistant Examiner(s): WHITE, DENNIS MICHAEL
• Application Number:
  US11/436,923
• Publication Number:
  US 20070154356A1
• Time in Patent Office: 2,015 Days
• Field of Search: 422/55, 422/82.05, 422/82.09, 422/102, 422/52, 422/82.08, 422/82.11, 422/99, 422/407, 435/7.9, 435/164, 435/165, 435/283.1, 435/287.1, 435/287.2, 435/4, 435/5, 435/7.2, 356/326, 356/128, 356/244, 356/246, 356/300, 356/414, 356/416, 356/445, 436/149, 436/164, 436/172, 436/174, 436/518, 436/805, 436/809, 204/403.01, 221/1, 359/321, 369/112.07, 382/133, 430/290, 430/321, 506/3, 506/39, 526/318.2, 526/319, 250/458.1, 250/559.29, 250/574, 385/12, 385/129, 385/130, 385/14, 385/37
• US Class Current: 436/164
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/774 the reagent being on a grating or periodic structure
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

A microplate for use within an interrogation system and a method of using the microplate are disclosed. The microplate contains within the bottom of each well, an optical waveguide grating based sensor. Approximate to each sensor is a mask having an aperture of predetermined size. The aperture regulates the light that enters and exits the sensor upon successive scans and ensures repeatable readings from the sensor. In an extended embodiment, a method of detection is disclosed that utilizes a launch and receive system while employing the aforementioned microplate.

Claims

1. A microplate comprising:

a frame that forms sidewalls of a plurality of wells, the frame having a bottom surface that interconnects said wells;

a first layer that forms a bottom of the plurality of wells, the layer having a top surface and a bottom surface, wherein said frame and said layer are attached such that said top surface of said layer contacts and bonds to said bottom surface of said frame;

a plurality of waveguide grating based biosensors, each waveguide grating based biosensor is located within the bottom of each well; and

a second layer comprising a mask attached to and in contact with the bottom surface of the first layer, said mask having a plurality of apertures that are aligned with the plurality of waveguide grating based biosensors, each aperture being smaller than a grating in each waveguide grating based biosensor, and wherein the mask is attached to the bottom surface of the first layer by an adhesive, a chemical bond, or a silk screen bond.

2. The microplate of claim 1 wherein said aperture is circular.

3. The microplate of claim 1 further comprising an array of 96 wells.

4. A method of interrogation comprising:

providing a microplate having a plurality of wells formed therein, where each well has a waveguide grating based biosensor in a bottom portion thereof and a mask attached to and in direct contact with the waveguide grating based biosensor, where the mask has an aperture that is aligned with the waveguide grating based biosensor, where the aperture is smaller than a grating in the waveguide grating based biosensor, and where the mask is attached to the waveguide grating based biosensor by an adhesive, a chemical bond, or a silk screen bond;

using a launch system to direct a light beam through the aperture toward the waveguide grating based biosensor, and wherein the light beam overfills the aperture in the mask; and

using a receive system to receive and interrogate the light beam that is reflected from the waveguide grating based biosensor.

5. The method of claim 4, wherein the width of said light beam is larger than a diameter of said aperture.

6. The method of claim 5, further comprising the step of using the launch system to direct another light beam through the aperture toward the waveguide grating based biosensor, where the aperture ensures that the another light beam hits a same region of the grating in the waveguide grating based biosensor as was done by the light beam during the first using step even when the microplate shifted relative to the launch system, where the width of the another light beam is larger than the diameter of said aperture.

7. A microplate comprising:

a frame that forms sidewalls of a plurality of wells, the frame having a bottom surface;

a layer that forms a bottom of the plurality of wells, the layer having a top surface and a bottom surface, wherein the frame and the layer are attached such that the top surface of the layer contacts and bonds to the bottom surface of the frame;

a plurality of waveguide grating based biosensors, each waveguide grating based biosensor is located within the bottom of each well; and

a mask in contact with and directly bonded to the bottom surface of the layer, the mask having a plurality of apertures that are aligned with the plurality of waveguide grating based biosensors, each aperture being smaller than a grating in each waveguide grating based biosensor, and wherein the mask is directly bonded to the bottom surface of the layer by an adhesive, a chemical bond, or a silk screen bond.

8. A microplate article comprising:

a plate having a plurality of parallel holes therethrough;

a base attached to a first face of the plate, the attached base creates a closed-end well from each hole;

a waveguide grating biosensor within each well on the base; and

a mask bonded to a face of the base opposite the attached plate, the mask having a light passable aperture aligned with each waveguide grating biosensor, and each light passable aperture having a facial area less than the facial area of the aligned grating, and wherein the mask is bonded to the face of the base opposite the attached plate by an adhesive, a chemical bond, or a silk screen bond.

9. The microplate article of claim 8 wherein the aperture comprises a pair of apertures.

10. An interrogation system for interrogating a microplate, the system comprising:

an optical source and receiver, the source emits an optical beam to the microplate and receives an optical beam from the microplate, the microplate comprises;

a frame that forms sidewalls of a plurality of wells, the frame having a bottom surface that interconnects the wells;

a first layer having a top surface and a bottom surface, the top surface is bonded to the bottom surface of the frame and seals one end of the wells;

at least one waveguide grating biosensor being located within at least one well; and

a second layer comprising a mask attached to and in contact with the bottom surface of the first layer, the mask having at least one aperture aligned with at least one waveguide grating biosensor, the aperture having a smaller area than the grating of the aligned waveguide grating biosensor, and the mask is attached to the first layer by an adhesive, a chemical bond, or a silk screen bond.