RNA detection assays

• US 8,063,184 B2
• Filed: 10/05/2010
• Issued: 11/22/2011
• Est. Priority Date: 11/29/1996
• Status: Expired due to Fees

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Third Wave Technologies Incorporated (Hologic Incorporated)
• Inventors: Allawi, Hatim, Argue, Brad T., Bartholomay, Christian Tor, Chehak, LuAnne, Curtis, Michelle L., Eis, Peggy S., Hall, Jeff G., Ip, Hon S., Ji, Lin, Kaiser, Michael, Kwiatkowski, Robert W. Jr., Lukowiak, Andrew A., Lyamichev, Victor, Lyamicheva, Natalie E., Ma, WuPo, Neri, Bruce P., Olson, Sarah M., Olson-Munoz, Marilyn C., Schaefer, James J., Skrzypczynski, Zbigniev, Takova, Tsetska Y., Thompson, Lisa C., Vedvik, Kevin L.
• Primary Examiner(s): Chunduru, Prabha
• Application Number:
  US12/898,490
• Publication Number:
  US 20110104682A1
• Time in Patent Office: 413 Days
• Field of Search: 530/350, 435/320.1
• US Class Current: 530/350
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: C12N 9/22 Ribonucleases RNAses, DNAses catalytic nucleic acids C12N15/113
• CPC Class Codes: C12Q 1/6823 Release of bound markers
• CPC Class Codes: C12Q 1/683 involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
• CPC Class Codes: C12Q 2561/109 Invader technology
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Claims

1. A kit comprising a composition comprising the recombinant polypeptide of SEQ ID NO:

2668.

2. The kit of claim 1, further comprising at least one nucleic acid cleavage substrate.

3. The kit of claim 2, further comprising at least one RNA capable of hybridizing to said nucleic acid cleavage substrate.

4. The kit of claim 2, further comprising a labeled oligonucleotide.

5. The kit of claim 2, further comprising an invasive oligonucleotide.