Nucleotide and amino acid sequences encoding an exported protein 1 derived from Plasmodium vivax and uses thereof

• US 8,063,193 B2
• Filed: 03/27/2009
• Issued: 11/22/2011
• Est. Priority Date: 03/27/2009
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Birkenmeyer, Larry G., Dawson, George J., Desai, Suresh M., Dille, Bruce J., Muerhoff, Anthony S., Coffey, Ruthie E.
• Primary Examiner(s): Minnifield, Nita M
• Assistant Examiner(s): Gangle, Brian J
• Application Number:
  US12/412,529
• Publication Number:
  US 20100247576A1
• Time in Patent Office: 970 Days
• Field of Search: None
• US Class Current: 536/23.7
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 33/02 Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/445 Plasmodium
• CPC Class Codes: G01N 2333/445 Plasmodium
• CPC Class Codes: G01N 33/56905 Protozoa
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention is directed to novel polynucleotides and polypeptides directed to EXP1 of Plasmodium vivax, and methods of using these polynucleotides and polypeptides in the detection of P. vivax antibodies or anti-P. vivax antibodies in a subject. The invention finds particular useful application in identifying recent exposure to P. vivax.

Claims

1. An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide, wherein the amino acid sequence of said polypeptide has at least 70% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:

2, SEQ ID NO;

3 and amino acids 2-50 of SEQ ID NO;

2.

3. The isolated nucleic acid of claim 1, wherein said nucleic acid is isolated from Plasmodium vivax.

2. An isolated nucleic acid comprising or complementary to a nucleotide sequence having at least 70% identity to a nucleotide sequence comprising SEQ ID NO:

1.

4. A method of producing a protein comprising the steps of:

(a) isolating a nucleic acid comprising SEQ ID NO;

1;

(b) constructing a vector comprising the isolated nucleic acid operably linked to a regulatory sequence; and

(c) introducing said vector into a host cell for a time and under conditions sufficient for expression of said protein.

5. The method of claim 4, wherein the host cell is a eukaryotic cell or a prokaryotic cell.

6. A vector comprising a nucleic acid sequence comprising SEQ ID NO:

1, operably linked to a regulatory sequence.

7. A host cell comprising the vector of claim 6.