Methods of quantifying taste of compounds for food or beverages

• US 8,067,185 B2
• Filed: 11/13/2008
• Issued: 11/29/2011
• Est. Priority Date: 03/07/2001
• Status: Expired due to Fees

• Current Assignee: Senomyx Inc (DSM-Firmenich AG)
• Original Assignee: Senomyx Inc (DSM-Firmenich AG)
• Inventors: Adler, Jon Elliot, Xu, Hong, Staszewski, Lena, Li, Xiaodong, Zoller, Mark, O'Connell, Shawn, Zozulya, Sergey, Echeverri, Fernando
• Primary Examiner(s): Landsman; Robert
• Application Number:
  US12/270,841
• Publication Number:
  US 20090221002A1
• Time in Patent Office: 1,111 Days
• Field of Search: None
• US Class Current: 435/7.1
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A61P 27/00 Drugs for disorders of the senses
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• CPC Class Codes: C12Q 2600/124 Animal traits, i.e. production traits, including athletic performance or the like
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G01N 2333/4719 G-proteins
• CPC Class Codes: G01N 2333/705 Assays involving receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: G01N 2333/726 G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
• CPC Class Codes: G01N 2500/04 Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
• CPC Class Codes: G01N 33/5008 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
• CPC Class Codes: G01N 33/5041 involving analysis of members of signalling pathways
• CPC Class Codes: G01N 33/566 using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds

Abstract

Methods of quantifying the taste of compounds for food and beverages are provided. These methods comprise contacting the compounds with an isolated heteromeric receptor comprising at least one T1R1 polypeptide and at least one T1R3 polypeptide.

Claims

1. A method of quantifying the taste of individual compounds for food or beverage compositions comprising:

contacting said individual compounds for food or beverage with an isolated heteromeric receptor, said an isolated heteromeric receptor comprising at least one T1R1 polypeptide and at least one T1R3 polypeptide, wherein said T1R1 polypeptide is (i) encoded by a nucleic acid sequence comprising SEQ. ID. NO;

8, (ii) encoded by a nucleic acid sequence comprising a nucleic acid that hybridizes to SEQ. ID. NO;

8 under stringent hybridization conditions which are conducting the hybridization reaction at 42°

C. in a solution comprising 50% formamide, 5×

SSC, and 1% SDS and washing at 65°

C. in a solution comprising 0.2×

SSC and 0.1% SDS, or (iii) a T1R1 polypeptide possessing at least 95% sequence identity to the T1R1 polypeptide of SEQ. ID. NO;

5;

wherein said T1R3 polypeptide is (i) encoded by a nucleic acid sequence comprising SEQ. ID. NO;

9;

(ii) encoded by a nucleic acid sequence that hybridizes to SEQ. ID. NO;

9 under stringent hybridization conditions which are conducting the hybridization reaction at 42°

C. in a solution comprising 50% formamide, 5×

SSC, 1% SDS; and

washing at 65°

C. in a solution comprising 0.2×

SCC and 0.1% SDS, or (iii) a T1R3 polypeptide possessing at least 95% sequence identity to the T1R3 polypeptide of SEQ. ID. NO;

7;

and wherein said heteromeric receptor comprised of at least one T1R1 polypeptide and at least one T1R3 polypeptide specifically binds to a ligand that specifically binds to an endogenous (wild-type) human heteromeric T1R1/T1R3 receptor; and

quantifying the taste of said compounds.

2. The isolated heteromeric receptor of claim 1, wherein said T1R1 polypeptide has the sequence of SEQ. ID. NO:

5.

3. The isolated heteromeric receptor of claim 1, wherein said T1R1 polypeptide has an amino acid sequence that possesses at least 95% sequence identity to the polypeptide of SEQ. ID. NO:

5.

4. The isolated heteromeric receptor of claim 1, wherein said T1R1 polypeptide has an amino acid sequence that possesses at least 96% sequence identity to the polypeptide of SEQ. ID. NO:

5.

5. The isolated heteromeric receptor of claim 1, wherein said T1R1 polypeptide has an amino acid sequence that possesses at least 97% sequence identity to the polypeptide of SEQ. ID. NO:

5.

6. The isolated heteromeric receptor of claim 1, wherein said T1R1 polypeptide has an amino acid sequence that possesses at least 98% sequence identity to the polypeptide of SEQ. ID. NO:

5.

7. The isolated heteromeric receptor of claim 1, wherein said T1R1 polypeptide has an amino acid sequence that possesses at least 99% sequence identity to the polypeptide of SEQ. ID. NO:

5.

8. The isolated heteromeric receptor of claim 1, wherein said T1R1 polypeptide is encoded by the nucleic acid sequence of SEQ. ID. NO:

8.

9. The isolated heteromeric receptor of claim 1, wherein said T1R1 polypeptide is encoded by a nucleic acid sequence that hybridizes to SEQ ID NO:

8 under stringent hybridization conditions which are conducting the hybridization reaction at 42°

C. in a solution comprising 50% formamide, 5×

SSC, and 1% SDS and washing at 65°

C. in a solution comprising 0.2×

SSC and 0.1% SDS.

10. The isolated heteromeric receptor of claim 1, wherein said T1R3 polypeptide has the sequence of SEQ. ID. NO:

7.

11. The isolated heteromeric receptor of claim 1, wherein said T1R3 polypeptide has an amino acid sequence that possesses at least 95% sequence identity to the polypeptide of SEQ. ID. NO:

7.

12. The isolated heteromeric receptor of claim 1, wherein said T1R3 polypeptide has an amino acid sequence that possesses at least 96% sequence identity to the polypeptide of SEQ. ID. NO:

7.

13. The isolated heteromeric receptor of claim 1, wherein said T1R3 polypeptide has an amino acid sequence that possesses at least 97% sequence identity to the polypeptide of SEQ. ID. NO:

7.

14. The isolated heteromeric receptor of claim 1, wherein said T1R3 polypeptide has an amino acid sequence that possesses at least 98% sequence identity to the polypeptide of SEQ. ID. NO:

7.

15. The isolated heteromeric receptor of claim 1, wherein said T1R3 polypeptide has an amino acid sequence that possesses at least 99% sequence identity to the polypeptide of SEQ. ID. NO:

7.

16. The isolated heteromeric receptor of claim 1, wherein said T1R3 polypeptide is encoded by the nucleic acid sequence of SEQ. ID. NO:

9.

17. The isolated heteromeric receptor of claim 1, wherein said T1R3 polypeptide is encoded by a nucleic acid sequence that hybridizes to SEQ ID NO:

9 under stringent hybridization conditions which are conducting the hybridization reaction at 42°

C. in a solution comprising 50% formamide, 5×

SSC, and 1% SDS and washing at 65°

C. in a solution comprising 0.2×

SSC and 0.1% SDS.

18. The method of claim 1, wherein said T1R1 and T1R3 sequences are expressed in a cell.

19. The method of claim 18, wherein said cell is intact or permeabilized.

20. The method of claim 18, wherein said T1R1 and T1R3 receptor sequences are expressed on the surface of said cell.

21. The method of claim 18, wherein the cell is an endogenous taste cell.

22. The method of claim 21, wherein the cell is a taste cell present in foliate, circumvallate or fungiform papillae.

23. The method of claim 22, wherein the cell is a taste cell present in geschmackstreifen, oral cavity, gastrointestinal epithelium or epiglottis.