Rapid microbial detection and antimicrobial susceptibiility testing

• US 8,071,319 B2
• Filed: 01/28/2008
• Issued: 12/06/2011
• Est. Priority Date: 07/12/2003
• Status: Active Grant

• Current Assignee: Accelerate Diagnostics, Inc.
• Original Assignee: Accelr8 Technology Corporation
• Inventors: Metzger, Steven W., Howson, David C., Goldberg, David A., Buttry, Daniel A.
• Primary Examiner(s): LAM, ANN Y
• Application Number:
  US12/021,087
• Publication Number:
  US 20080241858A1
• Time in Patent Office: 1,408 Days
• Field of Search: None
• US Class Current: 435/7.2
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 20/00 Nanooptics, e.g. quantum optics or photonic crystals
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/18 Testing for antimicrobial activity of a material
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/561 Immunoelectrophoresis

Abstract

A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms.

Claims

1. A method for measuring the growth of a plurality of individual microorganisms in a sample comprising:

contacting said sample with a biosensor comprising;

a chamber having at least one detection surface configured to bind a plurality of said microorganisms wherein each said individual microorganism binds to said detection surface in spatially discrete sites;

an input port configured to transport a solution into the chamber; and

an output port configured to transport said solution out of said chamber;

allowing said individual microorganisms to grow on said detection surface for a first period of time; and

detecting growth of individual microorganisms on said detection surface as an indication of the presence of said microorganisms.

2. The method according to claim 1 wherein said allowing step comprises adding at least one bioactive agent to said chamber.

3. The method according to claim 1 wherein a plurality of bioactive agents are added to the chamber.

4. The method according to claim 2 wherein said agent(s) are added after a first growth period.

5. The method according to claim 3 wherein said agent(s) are added after a first growth period.

6. The method according to claim 2 further comprising measuring growth of said individual microorganisms prior to said addition of said agent(s);

and measuring growth of said individual microorganisms after a second growth period in the presence of said agent(s).

7. The method according to claim 3 further comprising measuring growth of said individual microorganisms prior to said addition of said agent(s);

and measuring growth of said individual microorganisms after a second growth period in the presence of said agent(s).