×

Synthesis of long-chain polyunsaturated fatty acids by recombinant cells

  • US 8,071,341 B2
  • Filed: 04/25/2011
  • Issued: 12/06/2011
  • Est. Priority Date: 04/22/2004
  • Status: Active Grant
First Claim
Patent Images

1. A yeast cell comprisingdocosapentaenoic acid and docosahexaenoic acid in an esterified form as part of triacylglycerols in the yeast cell;

  • a polynucleotide which encodes a Δ

    5 elongase operably linked to a promoter which directs expression of the polynucleotide in the yeast cell;

    a polynucleotide which encodes a Δ

    4 desaturase operably linked to a promoter which directs expression of the polynucleotide in the yeast cell;

    an exogenous polynucleotide which encodes a desaturase which desaturates an acyl-CoA substrate in a yeast cell; and

    a total fatty acid content in the yeast cell which includes 2.5% (w/w) C20 ω

    3 long-chain polyunsaturated fatty acids (LC-PUFAs) and 0.1% (w/w) docosapentaenoic acid.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×