Synthetic molecules that specifically react with target sequences

• US 8,071,789 B2
• Filed: 03/18/2009
• Issued: 12/06/2011
• Est. Priority Date: 10/21/1997
• Status: Expired due to Fees

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Tsien, Roger Y., Griffin, B. Albert
• Primary Examiner(s): Russel, Jeffrey E
• Application Number:
  US12/406,702
• Publication Number:
  US 20100317097A1
• Time in Patent Office: 993 Days
• Field of Search: None
• US Class Current: 549/3
• CPC Class Codes: C07F 9/74 Aromatic compounds
• CPC Class Codes: C07F 9/80 Heterocyclic compounds
• CPC Class Codes: G01N 33/532 Production of labelled immunochemicals

Abstract

The present invention features biarsenical molecules. Target sequences that specifically react with the biarsenical molecules are also included. The present invention also features kits that include biarsenical molecules and target sequences. Tetraarsenical molecules are also featured in the invention.

Claims

1. A kit comprising

a. a biarsenical molecule of the formula:

2. The kit of claim 1, wherein said target sequence comprises a Cys-Cys-X-Y-Cys-Cys sequence, wherein said X and Y are amino acids.

3. The kit of claim 1, wherein said target sequence is selected from the group consisting of SEQ ID NO. 1 and SEQ ID NO. 4.

4. The kit of claim 1, wherein said carrier molecule comprises a carrier polypeptide.

5. The kit of claim 4, wherein said target sequence is heterologous to said carrier polypeptide.

6. The kit of claim 1, wherein said biarsenical molecule specifically reacts with said target sequence and generates a detectable signal.

7. The kit of claim 6, wherein said detectable signal is a fluorescent signal.

8. A kit comprising

a. a biarsenical molecule of the formula:

9. The kit of claim 8, wherein said target sequence comprises four cysteines.

10. The kit of claim 8, wherein said target sequence is selected from the group consisting of SEQ ID NO. 1 and SEQ ID NO. 4.

11. The kit of claim 8, wherein said vector further comprises a nucleic acid sequence encoding a carrier polypeptide.

12. The kit of claim 11, wherein said carrier polypeptide is heterologous to said target sequence.

13. The kit of claim 8, wherein said biarsenical molecule specifically reacts with said target sequence and generates a detectable signal.

14. The kit of claim 13, wherein said detectable signal is a fluorescent signal.

15. A complex comprising a biarsenical molecule and a target sequence associated with a carrier polypeptide, said target sequence comprising one or more cysteines capable of reacting with said biarsenical molecule, said biarsenical molecule having the following formula:

16. The complex of claim 15, wherein said target sequence comprises four cysteines.

17. The complex of claim 15, wherein said target sequence is selected from the group consisting of SEQ ID NO. 1 and SEQ ID NO. 4.