System with extended range of molecular sensing through integrated multi-modal data acquisition
First Claim
1. A multi-modal data acquisition system for detecting target material on a biological reaction surface, the data acquisition system comprising:
- a radiation source for generating an incoming beam to illuminate the biological reaction surface, the incoming beam impinging on the biological reaction surface and producing a reflected beam, the reflected beam being produced by the incoming beam reflecting off the biological reaction surface;
a first detector for detecting a first signal from the illuminated biological reaction surface due to a label-free process, the reflected beam being directed to the first detector,a second detector for detecting a second signal from the illuminated biological reaction surface due to a labeled process; and
a processing system for receiving the first signal and the second signal and determining the presence or absence of the target material on the biological reaction surface using the first signal and the second signal.
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Abstract
A multi-modal data acquisition system for detecting target material on a biological reaction surface, the system comprising a radiation source for generating an incoming beam that impinges on the biological reaction surface at an oblique incidence angle and produces a reflected beam, an interferometric detector for detecting an interferometric signal from the illuminated surface, the reflected beam being directed to the interferometric detector, a fluorescence detector for detecting a fluorescence signal from the illuminated surface; the fluorescence detector being positioned to substantially minimize the incidence of the reflected beam; and a processing system for receiving the interferometric and fluorescence signals and determining the presence or absence of target material on the biological reaction surface. A reaction surface conditioned for the simultaneous collection of fluorescence, interferometric and other signals. A multi-modal data acquisition system for collecting and processing additional modes, including multiple interferometric, fluorescence and scattering channels.
238 Citations
34 Claims
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1. A multi-modal data acquisition system for detecting target material on a biological reaction surface, the data acquisition system comprising:
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a radiation source for generating an incoming beam to illuminate the biological reaction surface, the incoming beam impinging on the biological reaction surface and producing a reflected beam, the reflected beam being produced by the incoming beam reflecting off the biological reaction surface; a first detector for detecting a first signal from the illuminated biological reaction surface due to a label-free process, the reflected beam being directed to the first detector, a second detector for detecting a second signal from the illuminated biological reaction surface due to a labeled process; and a processing system for receiving the first signal and the second signal and determining the presence or absence of the target material on the biological reaction surface using the first signal and the second signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A multi-modal data acquisition system for detecting target material in a sample, the data acquisition system comprising:
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a biological compact disc having antibodies for reacting with the target material in the sample; a radiation source for generating an incoming beam to illuminate the biological compact disc, the incoming beam impinging on the biological compact disc at an oblique incidence angle and producing a reflected beam in the specular direction, the reflected beam being produced by the incoming beam reflecting off the biological compact disc; an interferometric detector for detecting an interferometric signal from the illuminated biological compact disc, the reflected beam being directed to the interferometric detector, a fluorescence detector for detecting a fluorescence signal from the illuminated biological compact disc;
the fluorescence detector being positioned to substantially minimize the incidence of the reflected beam on the fluorescence detector; andan oscilloscope connected to the interferometric detector for receiving the interferometric signal and producing an interferometric digital data stream therefrom, and connected to the fluorescence detector for receiving the fluorescence signal and producing a fluorescence digital data stream therefrom; and a computer connected to the oscilloscope for receiving the interferometric digital data stream and the fluorescence digital data stream, and determining the presence or absence of the target material on the biological compact disc using the interferometric digital data stream and the fluorescence digital data stream. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method of using a multi-modal data acquisition system for detecting target material on a biological compact disc, the method comprising:
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generating an incoming beam to illuminate the biological compact disc using a radiation source; directing the incoming beam to impinge on the biological compact disc to produce a reflected beam; collecting the reflected beam with an interferometric detector to produce an interferometric signal, collecting a fluorescence signal with a fluorescence detector, digitizing the interferometric signal to produce an interferometric digital data stream; digitizing the fluorescence signal to produce a fluorescence digital data stream; and correlating the interferometric digital data stream and the fluorescence digital data stream to determine the presence or absence of the target material on the biological compact disc. - View Dependent Claims (29, 30, 31, 32, 33, 34)
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Specification