Use of multipotent adult stem cells in treatment of myocardial infarction and congestive heart failure
First Claim
1. A method of providing cardiac muscle to a subject in need thereof, said method comprising administering to the subject, in an amount effective to provide new cardiac muscle, isolated expanded human multipotent non-ES, non-germ, and non-embryonic germ cells that express telomerase and can differentiate into ectodermal, endodermal, and mesodermal cell types, wherein the existing cardiac muscle is damaged by disease or injury, wherein said telomerase-expressing cells were expanded in cell culture, and wherein said telomerase-expressing cells have undergone 10-40 cell doublings in culture.
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Abstract
The invention provides isolated stem cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. Also provided are methods of isolation and culture, as well as therapeutic uses for the isolated cells in cardiac indications.
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Citations
16 Claims
- 1. A method of providing cardiac muscle to a subject in need thereof, said method comprising administering to the subject, in an amount effective to provide new cardiac muscle, isolated expanded human multipotent non-ES, non-germ, and non-embryonic germ cells that express telomerase and can differentiate into ectodermal, endodermal, and mesodermal cell types, wherein the existing cardiac muscle is damaged by disease or injury, wherein said telomerase-expressing cells were expanded in cell culture, and wherein said telomerase-expressing cells have undergone 10-40 cell doublings in culture.
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2. A method of increasing cardiac muscle function in a subject with existing cardiac muscle, said method comprising administering to the subject, in an amount effective to increase cardiac muscle function, isolated expanded human multipotent non-ES, non-germ, and non-embryonic germ cells that express telomerase and can differentiate into ectodermal, endodermal, and mesodermal cell types, wherein the existing cardiac muscle is damaged by disease or injury, wherein said telomerase-expressing cells were expanded in cell culture, and wherein said telomerase-expressing cells have undergone 10-40 cell doublings in culture.
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3. A process for producing cardiac muscle cells in a subject, said method comprising administering to the subject, in proximity to existing cardiac muscle and in an amount effective to produce cardiac muscle cells, isolated expanded human multipotent non-ES, non-germ, and non-embryonic germ cells that express telomerase and can differentiate into ectodermal, endodermal, and mesodermal cell types, wherein the existing cardiac muscle is damaged by disease or injury, wherein said telomerase-expressing cells were expanded in cell culture, and wherein said telomerase-expressing cells have undergone 10-40 cell doublings in culture.
Specification