Administration of cells and cellular extracts for rejuvenation

• US 8,075,920 B2
• Filed: 05/11/2007
• Issued: 12/13/2011
• Est. Priority Date: 05/11/2006
• Status: Active Grant

• Current Assignee: Regenics AS
• Original Assignee: Regenics AS
• Inventors: Gammelsaeter, Runhild, Remmereit, Jan
• Primary Examiner(s): BERTOGLIO, VALARIE E
• Application Number:
  US11/801,778
• Publication Number:
  US 20090175927A1
• Time in Patent Office: 1,677 Days
• Field of Search: 424/523
• US Class Current: 424/523
• CPC Class Codes: A61K 31/7088 Compounds having three or more nucleosides or nucleotides
• CPC Class Codes: A61K 35/36 Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells islets of Langerhans A61K35/39
• CPC Class Codes: A61K 35/60 Fish, e.g. seahorses; Fish eggs
• CPC Class Codes: A61K 38/02 Peptides of undefined number of amino acids; Derivatives thereof
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 8/606 Nucleosides; Nucleotides; Nucleic acids
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/65 Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/9789 Magnoliopsida [dicotyledons]
• CPC Class Codes: A61K 8/981 of mammals or bird
• CPC Class Codes: A61K 8/982 Reproductive organs; Embryos, Eggs
• CPC Class Codes: A61K 8/987 of species other than mammals or birds
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/16 Emollients or protectives, e.g. against radiation
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 39/06 Free radical scavengers or antioxidants
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61Q 19/00 : Preparations for care of the skin
• CPC Class Codes: A61Q 19/004 : Aftersun preparations
• CPC Class Codes: A61Q 19/06 : for countering cellulitis
• CPC Class Codes: A61Q 19/08 : Anti-ageing preparations

Abstract

The invention describes methods and agents for improving cosmetic appearance, for promoting, improving or restoring health of cells and tissues, preferably skin, and more preferably, for restoring aged or damaged skin to a healthy appearance. In some embodiments, the invention relates to compositions of cells, eggs, cell extracts, egg extracts, and extract components such as purified nucleic acids, polypeptides, lipids, carbohydrates or other natural products.

Claims

1. A composition comprising an isolated water soluble cytoplasmic fraction of salmon eggs comprising protein and messenger ribonucleic acid, wherein the protein content of said cytoplasmic fraction is about 100 to 380 mg/ml and a lipid component from a different source than said salmon eggs.

2. The composition of claim 1, further comprising an epigenetic inhibitor.

3. The composition of claim 1, wherein said composition is provided in a cream, gel, emulsion, ointment, spray, powder or lotion.

4. A composition comprising an isolated water soluble cytoplasmic fraction of salmon eggs comprising protein and messenger ribonucleic acid, wherein the protein content of said cytoplasmic fraction is about 100 to 380 mg/ml, in a cream, gel, spray, emulsion, solid, plastic or matrix, ointment, powder or lotion suitable for topical administration.

5. The composition of claim 4 wherein said composition further comprises purified or synthetic nucleic acid sequences, polypeptides, or natural products or combinations thereof.

6. The composition of claim 4 wherein said composition further comprises an epigenetic inhibitor.

7. The composition of claim 4, wherein said composition further comprises a lipid or protein fraction from a source other than said salmon eggs.

8. The composition of claim 7 wherein the lipid fraction comprises squalene, an aliphatic wax, a sterol ester, a diol ester, a triglyceride, or a sterols or combinations thereof.

9. The composition of claim 4 wherein said composition further comprises keratin or filaggrin.

10. The composition of claim 4 wherein said composition further comprises glutamine, antiinfective agents, anti-inflammatory agents, antioxidants and/or nicotinamide.

11. The composition of claim 10 wherein said antioxidant is vitamin A, C, D, or E or combinations thereof.

12. The composition of claim 4, wherein said gel comprises a compound selected from the group consisting of hyaluronic acid and chitosan.

13. The composition of claim 4 wherein said spray is an aerosol.

14. The composition of claim 4 wherein said spray dries on the skin.

15. The composition of claim 4 wherein said spray composition comprises gel-forming components.

16. A composition for stimulating the growth of cells comprising an effective amount of an isolated water soluble cytoplasmic fraction of salmon eggs comprising protein and messenger ribonucleic acid, wherein the protein content of said cytoplasmic fraction is about 100 to 380 mg/ml and said cytoplasmic fraction causes an increase in expression of OCT4 and SOX2 gene expression when applied to 293T cells.