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Method of treatment of RNA sample

  • US 8,076,064 B2
  • Filed: 07/09/2005
  • Issued: 12/13/2011
  • Est. Priority Date: 07/09/2005
  • Status: Active Grant
First Claim
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1. A method of treating a sample of RNA, the method comprising:

  • a) contacting the sample of RNA with an analogous DNA set to provide a DNA/RNA duplex, wherein the analogous DNA set comprises at least one DNA oligonucleotide comprising at least ten contiguous nucleotides of a small RNA in said sample, except that uracils in the sequence of said small RNA are substituted with thymines in said DNA oligonucleotide;

    b) contacting the DNA/RNA duplex with an enzyme having an RNase H activity to provide a digested RNA sample; and

    c) analyzing said digested RNA sample to detect said small RNA.

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