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System and method of capturing multiple source excitations from a single location on a flow channel

  • US 8,077,310 B2
  • Filed: 08/30/2007
  • Issued: 12/13/2011
  • Est. Priority Date: 08/30/2006
  • Status: Active Grant
First Claim
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1. A system for capturing multi source excitations from a single location on a flow channel comprising:

  • a light subsystem that emits light onto a single location on a flow channel, wherein the light subsystem includes a first excitation source that emits light at a first wavelength and a second excitation source that emits light at a second wavelength that is different from the first wavelength, wherein the light subsystem modulates between the following excitation source operating modes;

    a first excitation source operating mode, wherein only one of the first and second excitation sources emits light onto the single location on the flow channel; and

    a second excitation source operating mode, wherein both the first and second excitation sources emit light onto the single location on the flow channel;

    a detector subsystem to that detects light emitted from the single location on the flow channel and generates a composite signal; and

    a processor coupled to the detector subsystem and adapted to separate the composite signal into composite mode signals each corresponding to a respective excitation source operating mode, wherein the processor is adapted to separate a composite mode signal into component mode signals corresponding to at least two overlapping emission spectra of fluorophores.

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