System and method of capturing multiple source excitations from a single location on a flow channel
First Claim
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1. A system for capturing multi source excitations from a single location on a flow channel comprising:
- a light subsystem that emits light onto a single location on a flow channel, wherein the light subsystem includes a first excitation source that emits light at a first wavelength and a second excitation source that emits light at a second wavelength that is different from the first wavelength, wherein the light subsystem modulates between the following excitation source operating modes;
a first excitation source operating mode, wherein only one of the first and second excitation sources emits light onto the single location on the flow channel; and
a second excitation source operating mode, wherein both the first and second excitation sources emit light onto the single location on the flow channel;
a detector subsystem to that detects light emitted from the single location on the flow channel and generates a composite signal; and
a processor coupled to the detector subsystem and adapted to separate the composite signal into composite mode signals each corresponding to a respective excitation source operating mode, wherein the processor is adapted to separate a composite mode signal into component mode signals corresponding to at least two overlapping emission spectra of fluorophores.
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Abstract
The invention includes a system and a method for capturing multi source excitations from a single location on a flow channel. The system preferably includes a light subsystem that emits light onto a single location on a flow channel, a detector subsystem to detect light emitted from the single location on the flow channel, and a processor to separate the detected light. The method preferably includes emitting light onto a single location on a flow channel, detecting light emitted from the single location on the flow channel, and separating the detected light.
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Citations
18 Claims
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1. A system for capturing multi source excitations from a single location on a flow channel comprising:
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a light subsystem that emits light onto a single location on a flow channel, wherein the light subsystem includes a first excitation source that emits light at a first wavelength and a second excitation source that emits light at a second wavelength that is different from the first wavelength, wherein the light subsystem modulates between the following excitation source operating modes; a first excitation source operating mode, wherein only one of the first and second excitation sources emits light onto the single location on the flow channel; and a second excitation source operating mode, wherein both the first and second excitation sources emit light onto the single location on the flow channel; a detector subsystem to that detects light emitted from the single location on the flow channel and generates a composite signal; and a processor coupled to the detector subsystem and adapted to separate the composite signal into composite mode signals each corresponding to a respective excitation source operating mode, wherein the processor is adapted to separate a composite mode signal into component mode signals corresponding to at least two overlapping emission spectra of fluorophores. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for capturing multi source excitations from a single location on a flow channel comprising the steps of:
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emitting light onto a single location on a flow channel, including emitting light from a first excitation source at a first wavelength and emitting light from a second excitation source at a second wavelength that is different from the first wavelength, wherein the step of emitting light includes modulating between the following excitation source operating modes; in a first excitation source operating mode, emitting light onto the single location on the flow channel from only one of the first and second excitation sources; and in a second excitation source operating mode, emitting light onto the single location on the flow channel from both the first and second excitation sources; detecting light emitted from the single location on the flow channel and generating a composite signal; separating the composite signal into two or more composite mode signals, wherein each composite mode signal corresponds to a respective excitation source operating mode, and separating one or more of the composite mode signals into component mode signals corresponding to at least two overlapping emission spectra of fluorophores. - View Dependent Claims (14, 15, 16, 17, 18)
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Specification