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Method and apparatus determining the isoelectric point of charged analyte

  • US 8,080,145 B2
  • Filed: 01/12/2004
  • Issued: 12/20/2011
  • Est. Priority Date: 01/15/2003
  • Status: Expired due to Fees
First Claim
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1. A method of determining the isoelectric point of a charged analyte comprising:

  • focusing a charged analyte in a flowing liquid in an electric field gradient to form in the flowing liquid a focused band of the charged analyte at a first stable position in the electric field gradient;

    changing the pH of the flowing liquid at least once by an amount sufficient to change the position of the focused band of the charged analyte within the electric field gradient to a second stable position in the electric field gradient;

    obtaining pH and corresponding position data for the charged analyte, comprising determining the pH of the flowing liquid and the corresponding position of the focused band of the charged analyte at a plurality of band positions within the electric field gradient; and

    determining the isoelectric point of the charged analyte based on the pH and corresponding position data.

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