Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
First Claim
Patent Images
1. A process for producing a substantially purified human growth hormone (hGH) conjugated to a water soluble polymer at position 35 of SEQ ID NO:
- 2, comprising;
a) culturing recombinant host cells capable of producing hGH comprising one or more non-naturally encoded amino acid(s) in a liquid nutrient medium containing the non-naturally encoded amino acid(s) under conditions which favor growth;
b) producing hGH comprising the non-naturally encoded amino acid(s) in said cells; and
c) producing substantially purified hGH polypeptide by conjugating said non-naturally encoded amino acid(s) in said hGH via an oxime bond to a water soluble polymer, wherein the non-naturally encoded amino acid has the structure;
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules.
-
Citations
8 Claims
-
1. A process for producing a substantially purified human growth hormone (hGH) conjugated to a water soluble polymer at position 35 of SEQ ID NO:
- 2, comprising;
a) culturing recombinant host cells capable of producing hGH comprising one or more non-naturally encoded amino acid(s) in a liquid nutrient medium containing the non-naturally encoded amino acid(s) under conditions which favor growth;
b) producing hGH comprising the non-naturally encoded amino acid(s) in said cells; and
c) producing substantially purified hGH polypeptide by conjugating said non-naturally encoded amino acid(s) in said hGH via an oxime bond to a water soluble polymer, wherein the non-naturally encoded amino acid has the structure;
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- 2, comprising;
Specification