Methods for forming small-volume electrical contacts and material manipulations with fluid microchannels

• US 8,083,915 B2
• Filed: 10/19/2007
• Issued: 12/27/2011
• Est. Priority Date: 02/04/1999
• Status: Active Grant

• Current Assignee: UT-Battelle LLC
• Original Assignee: UT-Battelle LLC
• Inventors: Ramsey, J. Michael, Culbertson, Christopher T., Whitten, William B., Foote, Robert S., Jacobson, Stephen C.
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US11/875,031
• Publication Number:
  US 20090032399A1
• Time in Patent Office: 1,530 Days
• Field of Search: 204451-455, 204601-605, 422/99, 422/100, 422/502, 422/503
• US Class Current: 204/451
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: B01D 61/422 Electrodialysis
• CPC Class Codes: B01D 61/427 Electro-osmosis
• CPC Class Codes: B01D 61/445 with bipolar membranes; Water splitting
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: F04B 17/00 Pumps characterised by combination with, or adaptation to, specific driving engines or motors
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either ionic current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to a variety of processes, including electrokinetically induced pressure flow in a region of a microchannel that is not influenced by an electric field, sample concentration enhancement and injection, as well as improving the analysis of materials where it is desired to eliminate electrophoretic bias. Other applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids.

Claims

1. A device for the manipulation of liquid phase materials, comprising:

a substrate;

a channel disposed in said substrate and containing a material, said channel having first and second ends;

an electrode made of an electrically conducting material, said electrode being in electrical contact with said channel at a location along said channel that is intermediate the first and second ends thereof;

a coverplate;

said coverplate and said substrate being mated together such that the coverplate seals the channel wherein either said substrate or said coverplate is formed of a gas permeable material that is selected to facilitate the removal of electrochemically generated gases from said channel; and

means for applying an electric potential in a first segment of the channel between the first end of said channel and said electrode such that an electric field-free region is created in a second segment of said channel between said electrode and the second end, whereby transport of the material can be induced in the first and second segments of the channel.

2. A device as set forth in claim 1 wherein the coverplate is made of the gas permeable material and the electrode is formed on the substrate.

3. A device as set forth in claim 1 wherein the substrate is made of the gas permeable material and the electrode is formed on the substrate.

4. A device as set forth in claim 1 wherein the substrate is made of the gas permeable material and the electrode is formed on the coverplate.

5. A device as set forth in claim 1 wherein the cover plate is made of the gas permeable material and the electrode is formed on the coverplate.

6. A device as set forth in claim 1 wherein both the substrate and the coverplate are formed of the gas permeable material.

7. A method for the manipulation of liquid phase materials, comprising the steps of:

providing a microchannel device that includes a substrate having a channel formed therein, an electrode made of an electrically conducting material, said electrode being in electrical contact with said channel at a location along said channel that is intermediate first and second ends thereof, and a coverplate for sealing the channel, wherein either said substrate or said coverplate is formed of a gas permeable material that is selected to facilitate the removal of electrochemically generated gases from said channel;

providing a material in the channel; and

inducing transport of the material in a first segment of said channel between said electrode and the first end of said channel and creating an electric field-free region in a second segment of said channel between the electrode and the second end of said channel by applying an electric potential between the first end of said channel and said electrode, whereby the material is transported in the first and second segments of said channel.

9. A method as set forth in claim 7 or 8 wherein both the substrate and the coverplate are formed of the gas permeable material.

10. A method as set forth in claim 7 or 8 wherein the step of inducing transport of the material in the channel comprises the step of applying the electric potential with a polarity such that transport of the material proceeds in a direction from the first end of the channel toward the second end of the channel.

11. A method as set forth in claim 7 or 8 wherein the step of inducing transport of the material in the channel comprises the step of applying the electric potential with a polarity such that transport of the material proceeds in a direction from the second end of the channel toward the first end of the channel.

8. A method for the manipulation of liquid phase materials, comprising the steps of:

providing a microchannel device that includes a substrate having a channel formed therein, first and second electrodes made of an electrically conducting material, said electrodes being in electrical contact with said channel at spaced apart locations along said channel that are intermediate first and second ends thereof, and a coverplate for sealing the channel, wherein either said substrate or said coverplate is formed of a gas permeable material that is selected to facilitate the removal of electrochemically generated gases from said channel;

providing a material in the channel; and

inducing transport of the material in a first segment of said channel by applying an electric potential between said first and second electrodes and creating electric field-free regions in a second segment of said channel between said first electrode and the first end of said channel and in a third segment of said channel between the second electrode and the second end of said channel,

whereby the material is transported in the first, second, and third segments.

12. A device for the manipulation of liquid phase materials, comprising:

a substrate;

a channel disposed in said substrate and containing a first material, said channel having first and second ends;

first and second electrodes each made of an electrically conducting material, said electrodes being in electrical contact with said channel at spaced apart locations along said channel that are intermediate the first and second ends thereof;

a coverplate;

said coverplate and said substrate being mated together such that the coverplate seals the channel, said substrate or said coverplate being formed of a gas permeable material that is selected to facilitate the removal of electrochemically generated gases from said channel; and

means for applying an electric potential in a first segment of the channel between the first and second electrodes such that electric field-free regions are created in a second segment of the channel between the first end of the channel and the first electrode and in a third segment of the channel between the second electrode and the second end of the channel, whereby transport of the first material can be induced in the first segment of the channel, in the second segment of said channel, and in the third segment of said first channel.

13. A device as set forth in claim 12 wherein both the substrate and the coverplate are formed of the gas permeable material.