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Method and a device for treating microparticles

  • US 8,084,271 B2
  • Filed: 10/20/2004
  • Issued: 12/27/2011
  • Est. Priority Date: 10/20/2003
  • Status: Active Grant
First Claim
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1. A method for handling microparticles (22) that bind a desired component from a sample, comprising:

  • (a) providing a vessel (26), wherein the vessel contains microparticles (22) in a solution (23), wherein the solution comprises a sample, wherein the sample comprises a desired biological component, wherein the desired component is selected from the group consisting of;

    a nucleic acid, a protein, a peptide, a cell organelle, a bacterium, a cell, and a virus;

    (b) incubating the microparticles and the sample in the vessel for a time sufficient for the microparticles to bind the desired component from the sample;

    (c) performing at least two treatment steps of the microparticles (22) in the vessel (26) with a magnetic tool (10) equipped with a protective shield (21) or coating wherein the microparticles are not moved to another vessel, and wherein the microparticles (22) are magnetic particles selected from the group consisting of;

    ferromagnetic particles, paramagnetic particles, and superparamagnetic particles, wherein the magnetic tool comprises a ferromagnetic sleeve;

    wherein the at least two treatment steps in the vessel comprise;

    at least one change of solutions (23) comprising removing one solution from the vessel and adding another solution to the vessel, wherein the microparticles (22) are collected and bound on the protective shield (21) or coating of the magnetic tool (10) during the at least one change of solutions (23);

    at least one mixing, wherein the microparticles (22) are mixed in the vessel (26) by moving the magnetic tool (10) in the solution (23) containing the microparticles thereby mixing the microparticles; and

    wherein the vessel is a tube or a well.

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