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Antisense antiviral compound and method for treating ssRNA viral infection

  • US 8,084,433 B2
  • Filed: 05/10/2006
  • Issued: 12/27/2011
  • Est. Priority Date: 09/16/2004
  • Status: Active Grant
First Claim
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1. A method of inhibiting replication of an infecting Dengue virus in a mammalian host cell, the method comprising administering to the infected host cell a virus-inhibitory amount of an antisense oligonucleotide analog compound characterized by:

  • (i) a nuclease-resistant backbone,(ii) capable of uptake by mammalian host cells,(iii) containing between 15-40 nucleotide bases,(iv) a substantially uncharged backbone, composed of morpholino subunits linked by phosphorodiamidate intersubunit linkages, joining a morpholino nitrogen of one subunit to a 5′

    exocyclic carbon of an adjacent subunit, and(v) having a targeting sequence of at least 15 subunits that is complementary to a region within the 5′

    -terminal 25 bases of SEQ ID NO;

    6, and by said administering, forming within the host cell, a heteroduplex structure (i) composed of the positive sense strand of the virus and the oligonucleotide compound, and (ii) characterized by a Tm of dissociation of at least 45°

    C. and disruption of a stem-loop secondary structure.

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