Polynucleotides for the detection of Escherichia coli O157:H7 and Escherichia coli O157:NM verotoxin producers
First Claim
1. An isolated polynucleotide of 50 nucleotides or less in length comprising the sequence as set forth in SEQ ID NO:
- 20 or the complement thereof for use in combination with a pair of primers capable of specifically amplifying a sequence as set forth in SEQ ID NO;
19 to detect E. coli O157;
H7 and/or E. coli O157;
NM which produces verotoxin.
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Accused Products
Abstract
Polynucleotide primers and probes for the specific detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in samples are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin. The primers and probes can be used in real-time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in a variety of situations and are capable of distinguishing E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin from other E. coli strains. Kits comprising the primers and probes are also provided.
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Citations
47 Claims
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1. An isolated polynucleotide of 50 nucleotides or less in length comprising the sequence as set forth in SEQ ID NO:
- 20 or the complement thereof for use in combination with a pair of primers capable of specifically amplifying a sequence as set forth in SEQ ID NO;
19 to detect E. coli O157;
H7 and/or E. coli O157;
NM which produces verotoxin. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
- 20 or the complement thereof for use in combination with a pair of primers capable of specifically amplifying a sequence as set forth in SEQ ID NO;
Specification