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Method and assembly for DNA isolation with dry reagents

  • US 8,088,576 B2
  • Filed: 04/28/2005
  • Issued: 01/03/2012
  • Est. Priority Date: 04/30/2004
  • Status: Active Grant
First Claim
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1. A method for DNA isolation while removing constituents that interfere with a subsequent PCR, the method comprising:

  • integrating materials in a closed analysis single-use unit which allows a sample with DNA to enter;

    releasing the DNA by using dry reagents stored in the unit in a form that is stable at room temperature;

    isolating the released DNA using DNA-binding substrates, wherein the DNA-binding substrates are magnetic beads;

    bringing a dry matrix, lying on a wall in a disintegration channel of the unit and including lysis reagents and the DNA-binding substrates, in contact with biological structures to disintegrate the biological structures containing the DNA, the magnetic beads being distributed in the dry matrix such that the DNAs released by contact with the lysis reagents are bound directly and simultaneously to the DNA-binding substrates; and

    collecting the substrates with the bound DNAs and transporting the collected substrates to a site of the PCR.

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