Methods of making small volume in vitro analyte sensors
First Claim
1. A method of manufacturing electrochemical sensors, the method comprising steps of:
- forming a plurality of working electrodes on a first electrode region of a substrate;
forming a plurality of counter electrodes on a second electrode region of the substrate;
forming a plurality of indicator electrodes on one of the first and second electrode regions;
disposing a spacer layer on one of the first and second electrode regions;
removing a portion of the spacer layer to define sample chamber regions;
folding the substrate with the spacer layer between the first electrode region and the second electrode region to form a layered structure; and
separating a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, at least one indicator electrode, at least one sample chamber region, and first and second lateral extensions from the sensor body flanking the sample chamber region to indicate to a user where the openings for the sample chamber are provided.
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Accused Products
Abstract
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
458 Citations
18 Claims
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1. A method of manufacturing electrochemical sensors, the method comprising steps of:
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forming a plurality of working electrodes on a first electrode region of a substrate; forming a plurality of counter electrodes on a second electrode region of the substrate; forming a plurality of indicator electrodes on one of the first and second electrode regions; disposing a spacer layer on one of the first and second electrode regions; removing a portion of the spacer layer to define sample chamber regions; folding the substrate with the spacer layer between the first electrode region and the second electrode region to form a layered structure; and separating a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, at least one indicator electrode, at least one sample chamber region, and first and second lateral extensions from the sensor body flanking the sample chamber region to indicate to a user where the openings for the sample chamber are provided. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification