Compliant microfluidic sample processing device

• US 8,092,759 B2
• Filed: 06/23/2010
• Issued: 01/10/2012
• Est. Priority Date: 07/05/2005
• Status: Active Grant

• Current Assignee: Diasorin Italia SPA
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Bedingham, William, Robole, Barry W.
• Primary Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US12/821,772
• Publication Number:
  US 20100266456A1
• Time in Patent Office: 566 Days
• Field of Search: 422/400, 422/401, 422/500, 422/501, 422/502, 422/506
• US Class Current: 422/500
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

Microfluidic sample processing disks with a plurality of fluid structures formed therein are disclosed. Each of the fluid structures preferably includes an input well and one or more process chambers connected to the input well by one or more delivery channels. The process chambers may be arranged in a compliant annular processing ring that is adapted to conform to the shape of an underlying thermal transfer surface under pressure. That compliance may be delivered in the disks of the present invention by locating the process chambers in an annular processing ring in which a majority of the volume is occupied by the process chambers. Compliance within the annular processing ring may alternatively be provided by a composite structure within the annular processing ring that includes covers attached to a body using pressure sensitive adhesive.

Claims

1. A microfluidic sample processing device comprising:

a body comprising first and second major surfaces;

a plurality of fluid structures, wherein each fluid structure of the plurality of fluid structures comprises;

a process chamber comprising a void formed through the first and second major surfaces of the body, and

a delivery channel in fluid communication with the process chamber, wherein the delivery channel comprises an inner channel formed in the second major surface of the body, an outer channel formed in the first major surface of the body, and a via formed through the first and second major surfaces of the body, wherein the via connects the inner channel to the outer channel;

a first cover attached to the first major surface of the body, the first cover defining the vias, the outer channels, and the process chambers in connection with the first major surface of the body;

a second cover attached to the second major surface of the body, the second cover defining the process chambers of the plurality of fluid structures in connection with the second major surface of the body,

wherein an inner edge of the second cover is located radially inwardly of an inner edge of the process chambers of the plurality of fluid structure, and wherein the inner edge of the second cover is located radially outwardly of the vias of the plurality of fluid structures; and

a central cover attached to the second major surface of the body, the central cover defining the inner channels and the vias in connection with the second major surface of the body,

wherein the second cover comprises a metal, and wherein the central cover is formed from one or more materials that are less thermally conductive than the second cover.

2. The microfluidic sample processing device of claim 1, wherein an outer edge of the central cover is located radially outwardly of the vias of the plurality of fluid structures.

3. The microfluidic sample processing device of claim 1, wherein an outer edge of the central cover is located radially inwardly of the inner edge of the second cover.

4. The microfluidic sample processing device of claim 1, wherein an outer edge of the central cover and the inner edge of the second cover define a junction located radially outside of the vias of the plurality of fluid structures.

5. The microfluidic sample processing device of claim 1, wherein the inner channel of at least one of the plurality of fluid structures extends along a line that is not coincident with a radius defined by a center of the sample processing device.

6. The microfluidic sample processing device of claim 1, wherein each fluid structure further comprises an input well located radially inwardly of the process chamber and connected to the inner channel.

7. The microfluidic sample processing device of claim 6, wherein the input wells of the plurality of the fluid structures are located within raised structures extending above the first major surface of the body, wherein each raised structure comprises two or more of the input wells.

8. The microfluidic sample processing device of claim 6, wherein each input well of the plurality of fluid structures is located at a different radial position and a different angular position than at least one adjacent input well.

9. The microfluidic sample processing device of claim 6, wherein the process chamber of each of the plurality of fluid structures is rotationally offset from its input well.

10. The microfluidic sample processing device of claim 6, wherein the input wells comprise voids formed through the first and second major surfaces of the body, and wherein the central cover defines ends of the input wells on the second major surface of the body.

11. The microfluidic sample processing device of claim 1, wherein the first cover is configured to transmit electromagnetic radiation of selected wavelengths into and/or out of the process chambers of the plurality of fluid structures.

12. The microfluidic sample processing device of claim 1, wherein the central cover is formed of a polymeric material.

13. The microfluidic sample processing device of claim 1, wherein the process chambers of the plurality of fluid structures form an annular processing ring that comprises a compliant structure in which the independent volumes of the plurality of process chambers maintain a fluidic integrity when a portion of the annular processing ring is deflected in a direction normal to the first and second major surfaces of the body.

14. The microfluidic sample processing device of claim 1, wherein the process chambers of the plurality of fluid structures form an annular processing ring, and further comprising one or more orphan chambers located within the annular processing ring, wherein each orphan chamber comprises a void or depression in the body.

15. The microfluidic sample processing device of claim 1, wherein the vias and the process chambers of the plurality of fluid structures define annular rings on the body.