Biological sample reaction chip and biological sample reaction method

• US 8,092,999 B2
• Filed: 01/27/2009
• Issued: 01/10/2012
• Est. Priority Date: 02/01/2008
• Status: Active Grant

• Current Assignee: Seiko Epson Corporation (Seiko Group)
• Original Assignee: Seiko Epson Corporation (Seiko Group)
• Inventors: Takagi, Fumio
• Primary Examiner(s): Kim, Young J
• Application Number:
  US12/360,136
• Publication Number:
  US 20090197274A1
• Time in Patent Office: 1,078 Days
• Field of Search: None
• US Class Current: 435/6.1
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/14 Means for pressure control
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

A biological sample reaction chip, including: a plurality of reaction containers; a reaction liquid introduction channel having a reaction liquid supply opening at a first end and an evacuation opening at a second end; and a reaction liquid quantifying channel, a third end of which is connected to one of the reaction containers, and a fourth end of which is connected to the reaction liquid introduction channel, wherein an interior of each of the reaction containers is coated with a reagent that is necessary for a reaction.

Claims

1. A biological sample reaction method using a biological sample reaction chip,

the biological sample reaction chip including:

a plurality of reaction containers;

a reaction liquid introduction channel having a reaction liquid supply opening at a first end and an evacuation opening at a second end; and

a plurality of reaction liquid quantifying channels communicating with the reaction containers, respectively, each of the reaction liquid quantifying channels having a third end connected to one of the reaction containers, a fourth end connected to the reaction liquid introduction channel, and a volume which is bigger than a volume of the reaction container,

an interior of each of the reaction containers being coated with a reagent that is necessary for a reaction,

the biological sample reaction method comprising;

reducing the pressure inside the reaction containers, the reaction liquid quantifying channels and the reaction liquid introduction channel to a predetermined pressure;

filling a reaction liquid into the reaction liquid introduction channel via the reaction liquid supply opening;

introducing the reaction liquid into the reaction liquid quantifying channels by reverting the pressure inside the reaction containers, the reaction liquid quantifying channels and the reaction liquid introduction channel to a pressure outside the chip;

removing the reaction liquid from the reaction liquid introduction channel;

introducing into the reaction containers the reaction liquid in the reaction liquid quantifying channels, by centrifugal force; and

carrying out a biological sample reaction process, wherein

in said filling the reaction liquid into the reaction liquid introduction channel, the reaction liquid lingers in the reaction liquid introduction channel without flowing into the reaction liquid quantifying channels,

in said introducing the reaction liquid into the reaction liquid quantifying channels, the reaction liquid is prevented from reaching the reaction containers.

2. The biological sample reaction method according to claim 1, wherein in the reduction of the pressure to the predetermined pressure, the pressure is reduced to a pressure ranging from 50% of the pressure outside the chip to less than the pressure outside the chip.

3. The biological sample reaction method according to claim 1, wherein

the biological sample reaction process is a process including nucleic acid amplification,

the reaction liquid has a target nucleic acid, an enzyme for amplifying nucleic acid and nucleotides, at predetermined concentrations, and

the reaction containers are coated beforehand with primers.