Method for producing biocomposite comprising collagen and polymer

• US 8,093,027 B2
• Filed: 09/13/2007
• Issued: 01/10/2012
• Est. Priority Date: 09/13/2006
• Status: Expired due to Fees

• Current Assignee: University of South Florida (State University System of Florida)
• Original Assignee: University of South Florida (State University System of Florida)
• Inventors: Turos, Edward, Koob, Thomas J., Greenhalgh, Kerriann Robyn
• Primary Examiner(s): NAFF, DAVID M
• Application Number:
  US11/901,209
• Publication Number:
  US 20080124371A1
• Time in Patent Office: 1,580 Days
• Field of Search: None
• US Class Current: 435/177
• CPC Class Codes: A61L 2300/64 Animal cells
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C08L 89/06 derived from leather or skin , e.g. gelatin

Abstract

The present invention concerns a biocomposite useful in artificial tissue replacement, methods for its production, and methods of use. The biocomposite can be implanted into humans or animals as an artificial tissue for treatment of a tissue defect. The biocomposite can be formed or manipulated into a desired shape for implantation.

Claims

1. A method for producing a synthetic biocomposite comprising a biotic material and an abiotic material, wherein the biotic material is collagen and the abiotic material is a polymer produced by polymerizing, monomers, wherein said method comprises polymerizing the monomers by emulsion polymerization to form an emulsion, and mixing the biotic material with the emulsion, followed by drying, thereby forming the synthetic biocomposite.

2. The method of claim 1, wherein the biotic material is collagen fibrils and the biocomposite is formed as a fiber.

3. The method of claim 1, wherein the abiotic material comprises a copolymer.

4. The method of claim 3, wherein the copolymer is ethyl acrylate-methyl methacrylate.

5. The method of claim 1, wherein the biocomposite further comprises a biologically active agent.

6. The method of claim 5, wherein the biologically active agent is a cell.

7. The method of claim 1, wherein polymerization is conducted under nitrogen.

8. The method of claim 1, wherein polymerization comprises emulsifying the monomer in water and a surfactant and polymerizing using a radical initiator.

9. The method of claim 8, wherein the polymerization is conducted under nitrogen at about 70°

C. for about 6 to 8 hours.

10. The method of claim 8, wherein the radical initiator is a peroxide, persulphate, alkyl hydroperoxide, thiosulphate, metabisulphite, or hydrosulphide.

11. The method of claim 1, wherein the monomer is acrylonitrile, acrylic acid, maleic acid, methyl acrylate, ethyl acrylate, butyl acrylate, butyl methacrylate, 2-ethylhexyl acrylate, methoxyethyl acrylate, dimethylamino acrylate, methacrylic acid, isobutyl methacrylate, 2-ethyl hexyl methacrylate, lauryl methacrylate, stearic methacrylate, dimethyl amino methacrylate, allyl methacrylate, 2-hydroxyethyl acrylate, 2-hydroxy propyl acrylate, 2-hydroxy ethyl methacrylate, modified acrylamide, modified methacrylamide glycidyl acrylate, styrene, vinyl acetate, vinyl toluene, or a synthetically modified acrylic.