Differentiation of primate pluripotent stem cells to hematopoietic lineage cells

• US 8,093,049 B2
• Filed: 03/26/2009
• Issued: 01/10/2012
• Est. Priority Date: 03/27/2008
• Status: Active Grant

• Current Assignee: Asterias Biotherapeutics Incorporated (Lineage Cell Therapeutics, Inc.)
• Original Assignee: Geron Corporation
• Inventors: Tseng, Suyi, Majumdar, Anish Sen, Nishimoto, Kevin, Reddy, Anita, Lebkowski, Jane S.
• Primary Examiner(s): LI, QIAN JANICE
• Application Number:
  US12/412,183
• Publication Number:
  US 20090246869A1
• Time in Patent Office: 1,020 Days
• Field of Search: None
• US Class Current: 435/377
• CPC Class Codes: A61K 2039/5154 Antigen presenting cells [APCs], e.g. dendritic cells or macrophages
• CPC Class Codes: C12N 2501/02 Compounds of the arachidonic acid pathway, e.g. prostaglandins, leukotrienes
• CPC Class Codes: C12N 2501/125 Stem cell factor [SCF], c-kit ligand [KL]
• CPC Class Codes: C12N 2501/155 Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
• CPC Class Codes: C12N 2501/165 Vascular endothelial growth factor [VEGF]
• CPC Class Codes: C12N 2501/22 Colony stimulating factors (G-CSF, GM-CSF)
• CPC Class Codes: C12N 2501/23 Interleukins [IL]
• CPC Class Codes: C12N 2501/24 Interferons [IFN]
• CPC Class Codes: C12N 2501/52 CD40, CD40-ligand (CD154)
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0639 Dendritic cells, e.g. Langherhans cells in the epidermis

Abstract

The invention provides methods of differentiating primate pluripotent stem cells into cells of hematopoietic lineage. The invention further provides hematopoietic lineage cells differentiated from primate pluripotent stem cells, as well as methods of using the same and kits comprising the same.

Claims

1. A method of differentiating primate pluripotent stem cells into immature dendritic cells comprising contacting the primate pluripotent stem cells with a plurality of exogenous cytokines comprising granulocyte-macrophage colony stimulating factor (GM-CSF) and bone morphogenic protein 4 (BMP-4).

2. The method of claim 1, further comprising contacting the primate pluripotent stem cells with one or more of the following:

vascular endothelial growth factor (VEGF), stem cell factor (SCF), fetal liver kinase ligand (FLT3L), thrombopoietin (TPO), interleukin 4 (IL-4) and interleukin 3 (IL-3).

3. The method of claim 1, wherein the primate pluripotent stem cells are human embryonic stem cells.

4. The method of claim 1, wherein the differentiation of the primate pluripotent stem cells into immature dendritic cells is performed under serum free conditions.

5. The method of claim 1, wherein the culture of the primate pluripotent stem cells and the differentiation of the primate pluripotent stem cells into immature dendritic cells is performed feeder free.

6. The method of claim 1, wherein the differentiation of the primate pluripotent stem cells into immature dendritic cells is performed stromal cell free.

7. The method of claim 1, further comprising making mature dendritic cells by contacting the immature dendritic cells with a maturation cocktail.

8. The method of claim 7, wherein the maturation cocktail comprises one or more of the following:

tumor necrosis factor α

(TNFα

), interleukin 1β

(IL1β

), interferon γ

(IFNγ

), prostaglandin E2 (PGE2), polyinosinic;

polycytidylic acid (POLY I;

C), interferon α

(IFNα

), CD40 ligand (CD40L) and granulocyte macrophage colony stimulating factor (GM-CSF).

9. The method of claim 7, further comprising contacting the mature dendritic cell with an antigen.

10. The method of claim 9, wherein the antigen is a nucleic acid molecule expressing an antigen.

11. The method of claim 10, wherein the nucleic acid molecule is an RNA molecule expressing an antigen.

12. The method of claim 9, wherein the antigen is a peptide.

13. The method of claim 9 further comprising contacting the mature dendritic cell with a radiation source.

14. A method of differentiating primate pluripotent stem cells into immature dendritic cells comprising:

a) forming an embryoid body from the primate pluripotent stem cells;

b) contacting the embryoid body from a) with bone morphogenic protein 4 (BMP-4), vascular endothelial growth factor (VEGF) and stem cell factor (SCF) to differentiate the primate pluripotent stem cells into mesoderm;

c) contacting the mesoderm of b) with vascular endothelial growth factor (VEGF), stem cell factor (SCF), and GM-CSF to differentiate the mesoderm into hematopoietic stem cells;

d) contacting the hematopoietic stem cells of c) with SCF and granulocyte macrophage colony stimulating factor (GM-CSF) to differentiate the hematopoietic stem cells into monocytes; and

e) contacting the monocytes of d) with granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin 4 (IL-4) to differentiate the monocytes into immature dendritic cells.

15. A method of differentiating in vitro a cell expressing stage specific embryonic antigen 3 (SSEA3), stage specific embryonic antigen 4 (SSEA4) and markers detectable using antibodies designated Tra-1-60, and Tra-1-81 into a cell expressing CD11c comprising contacting the cell expressing stage specific embryonic antigen 3 (SSEA3), stage specific embryonic antigen 4 (SSEA4) and markers detectable using antibodies designated Tra-1-60, and Tra-1-81 with a differentiation cocktail comprising:

GM-CSF and BMP-4.

16. The method of claim 15, further comprising contacting the cell expressing stage specific embryonic antigen 3 (SSEA3), stage specific embryonic antigen 4 (SSEA4) and markers detectable using antibodies designated Tra-1-60, and Tra-1-81 with VEGF.

17. The method of claim 15, further comprising contacting the cell expressing stage specific embryonic antigen 3 (SSEA3), stage specific embryonic antigen 4 (SSEA4) and markers detectable using antibodies designated Tra-1-60, and Tra-1-81 with SCF.