Pegylated human interferon polypeptides

• US 8,093,356 B2
• Filed: 10/26/2007
• Issued: 01/10/2012
• Est. Priority Date: 06/03/2005
• Status: Expired due to Fees

• Current Assignee: Ambrx, Inc. (Johnson & Johnson)
• Original Assignee: Ambrx, Inc. (Johnson & Johnson)
• Inventors: Kimmel, Bruce E., Cho, Ho Sung, Sim, Bee-Cheng, Litzinger, David C., Mariani, Roberto, Kraynov, Vadim, Knudsen, Nick, Daniel, Thomas O., Koder, Alan, Bussell, Stuart, Liu, Junjie, Miao, Zhenwei, Hays, Anna-Maria A., Morrow, Theresa
• Primary Examiner(s): Saoud, Christine J
• Assistant Examiner(s): Seharaseyon, Jegatheesan
• Application Number:
  US11/925,337
• Publication Number:
  US 20080132681A1
• Time in Patent Office: 1,537 Days
• Field of Search: None
• US Class Current: 530/351
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: A61P 37/08 Antiallergic agents antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 14/555 Interferons [IFN]
• CPC Class Codes: C07K 14/56 IFN-alpha

Abstract

Modified human interferon polypeptides and uses thereof are provided, including PEGylated human interferon polypeptides with one or more non-naturally encoded amino acids.

Claims

1. A human interferon alpha (hIFN) polypeptide comprising a non-naturally encoded amino acid at amino acid position 134 of the hIFNα

polypeptide in SEQ ID NO;

2 or the corresponding amino acid position in SEQ ID NO;

1, 3 or other known IFN polypeptide sequence, wherein the non-naturally encoded amino acid has the structure;

2. The hIFN polypeptide of claim 1, wherein the branched polymer is greater than 30 kDa.

3. The hIFN polypeptide of claim 1, wherein the branched polymer is between about 40 kDa and 50 kDa.

4. A method of making the hIFN polypeptide of claim 1, the method comprising contacting an isolated hIFN polypeptide comprising a non-naturally encoded amino acid with a water soluble branched polymer that reacts with the non-naturally encoded amino acid.

5. The hIFN polypeptide of claim 1 wherein the polypeptide comprises SEQ ID NO:

2.