Chalcone synthase dihydroflavonol 4-reductase and leucoanthocyanidine reductase from clover, medic ryegrass or fescue
First Claim
1. A nucleic acid construct comprisinga first nucleic acid portion encoding a chalcone synthase (CHS), said first nucleic acid portion comprising a nucleotide sequence selected from the group consisting of:
- (a1) Sequence ID No. 1; and
(b1) nucleotide sequences encoding Sequence ID No. 2; and
a second nucleic acid portion encoding anthocyanine reductase, said second nucleic acid portion comprising a nucleotide sequence selected from the group consisting of;
(a2) Sequence ID No. 9; and
(b2) nucleotide sequences encoding Sequence ID No. 10,said construct increasing the levels of both CHS and anthocyanine reductase when the construct is introduced into a plant cell.
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Abstract
The present invention relates to nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of, for example, flavonoid biosynthesis in plants, and more specifically the modification of the content of condensed tannins. In particularly preferred embodiments, the invention relates to the combinatorial expression of chalcone synthase (CHS) and/or dihydroflavonol 4-reductase (BAN) and/or leucoanthocyanidine reductase (LAR) in plants to modify, for example, flavonoid biosynthesis or more specifically the content of condensed tannins.
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Citations
16 Claims
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1. A nucleic acid construct comprising
a first nucleic acid portion encoding a chalcone synthase (CHS), said first nucleic acid portion comprising a nucleotide sequence selected from the group consisting of: -
(a1) Sequence ID No. 1; and (b1) nucleotide sequences encoding Sequence ID No. 2; and a second nucleic acid portion encoding anthocyanine reductase, said second nucleic acid portion comprising a nucleotide sequence selected from the group consisting of; (a2) Sequence ID No. 9; and (b2) nucleotide sequences encoding Sequence ID No. 10, said construct increasing the levels of both CHS and anthocyanine reductase when the construct is introduced into a plant cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A nucleic acid construct comprising
a first nucleic acid portion for reducing the amount of chalcone synthase (CHS), said first nucleic acid portion comprising a nucleotide sequence selected from the group consisting of: -
(A1) Sequence ID No. 1, and the complements thereof; (B1) nucleotide sequences encoding Sequence ID No. 2, and the complements thereof; and (C1) functionally active fragments of the sequences recited in (A1), and (B1), said functionally active fragments having a size of at least 60 nucleotides; and a second nucleic acid portion for reducing anthocyanine reductase, said second nucleic acid portion comprising a nucleotide sequence selected from the group consisting of; (A2) Sequence ID No. 9 and the complement thereof; (B2) nucleotide sequences encoding Sequence ID No. 10 and the complement thereof; and (C2) functionally active fragments of the sequences recited in (A12) and (B2), said functionally active fragments having a size of at least 60 nucleotides; said construct reducing the levels of both CHS and anthocyanine reductase when the construct is introduced into a plant cell. - View Dependent Claims (13, 14, 15, 16)
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Specification