Multipoint method for identifying hazardous agents

• US 8,094,294 B2
• Filed: 04/13/2009
• Issued: 01/10/2012
• Est. Priority Date: 06/30/2004
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Treado, Patrick J, Schweitzer, Robert, Demuth, Joseph E
• Primary Examiner(s): Evans, F. L.
• Application Number:
  US12/422,360
• Publication Number:
  US 20110299071A1
• Time in Patent Office: 1,002 Days
• Field of Search: None
• US Class Current: 356/73
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6402 Atomic fluorescence; Laser induced fluorescence
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04

Abstract

The invention relates to apparatus and methods for assessing occurrence of a hazardous agent in a sample by performing multipoint spectral analysis of the sample. Methods of employing Raman spectroscopy and other spectrophotometric methods are disclosed. Devices and systems suitable for performing such multipoint methods are also disclosed.

Claims

1. A method of assessing the occurrence of a hazardous agent in a sample located in a field of view, the method comprising:

irradiating said sample to thereby generate a first plurality of interacted photons from a first set of multiple points in the sample, wherein

said first plurality of interacted photons are selected from the group consisting of;

photons absorbed by said multiple points, photons reflected by said multiple points, photons emitted by said multiple points, and combinations thereof, and

wherein said first set of multiple points represent a portion of total points in the field of view;

assessing said first plurality of interacted photons to thereby generate a first spectroscopic data set representative of said sample; and

analyzing said first spectroscopic data set to thereby determine at least one of;

the presence of a hazardous agent in said sample and the absence of a hazardous agent in said sample.

2. The method of claim 1 wherein said first plurality of interacted photons are assessed using at least one of:

infrared spectroscopy, fluorescence spectroscopy, UV/visible spectroscopy, and combinations thereof.

3. The method of claim 1 wherein said hazardous agent comprises at least one chemical agent.

4. The method of claim 1 wherein said hazardous agent comprises at least one biological agent.

5. The method of claim 1 wherein said hazardous agent comprises at least one microorganism.

6. The method of claim 1 wherein said hazardous agent comprises at least one bacterium.

7. The method of claim 1 wherein said hazardous agent comprises at least one protozoan.

8. The method of claim 1 wherein said hazardous agent comprises at least one virus.

9. The method of claim 1 further comprising passing said first plurality of interacted photons through a filter.

10. The method of claim 9 wherein said filter is selected from the group consisting of:

Fabry Perot angle tuned filter, an acousto-optic tunable filter, a liquid crystal tunable filter, a Lyot filter, an Evans split element liquid crystal tunable filter, a Solc Liquid crystal tunable filter, and a liquid crystal Fabry Perot tunable filter, and combinations thereof.

11. The method of claim 1 further comprising passing said first plurality of interacted photons through an interferometer.

12. The method of claim 11 wherein said interferometer is selected from the group consisting of:

a polarization-independent imaging interferometer, a Michelson interferometer, a Sagnac interferometer, a Twynam-Green interferometer, a Mach-Zehnder interferometer, and a tunable Fabry Perot interferometer, and combinations thereof.

13. The method of claim 1 further comprising passing said first plurality of interacted photons through a dispersive spectrometer.

14. The method of claim 1 wherein said first plurality of interacted photons are collected using a device selected from the group consisting of:

a telescope, a macroscope, a microscope, an endoscope, a fiber optic array, and combinations thereof.

15. The method of claim 1 wherein said first set of multiple points have a defined geometric relationship.

16. The method of claim 15 wherein the geometric relationship of said first set of multiple points is defined based on a spectroscopic feature of the sample.

17. The method of claim 1 wherein interacted photons from at least three points is assessed.

18. The method of claim 1 wherein interacted photons from at least five points is assessed.

19. The method of claim 1 wherein the interacted photons from at least ten points is assessed.

20. The method of claim 1 wherein the interacted photons from at least fifty points is assessed.

21. The method of claim 1 further comprising:

illuminating said sample to thereby generate a second plurality of interacted photons from a second set of multiple points, wherein

said second plurality of interacted photons is selected from the group consisting of;

photons absorbed by said sample, photons reflected by said sample, photons emitted by said sample, and combinations thereof, and wherein

said second set of multiple points corresponds to at least one point in said first set of multiple points wherein said hazardous agent was detected; and

assessing said second plurality of interacted photons to thereby determine at least one of;

the presence of a hazardous agent and the absence of a hazardous agent.

22. The method of claim 1 wherein said analyzing comprises comparing said first spectroscopic data set to at least one reference data set corresponding to a known hazardous agent.

23. The method of claim 1 wherein at least three of the multiple points are collinear.

24. The method of claim 1 wherein at least three of the multiple points are colinear along a first line and wherein another at least three of the multiple points are colinear along a second line.

25. The method of claim 1 wherein at least four of the multiple points are radially equidistant from a central point.

26. The method of claim 1 wherein the field of view is in a microscopic field with the sample in the microscopic field, and the multiple points represent not more than 25% of the area of the microscopic field.

27. The method of claim 1 wherein the field of view is in a microscopic field with the sample in the microscopic field, and the multiple points represent not more than 5% of the area of the microscopic field.

28. The method of claim 1 wherein the field of view is in a microscopic field with the sample in the microscopic field, and the multiple points represent not more than 1% of the area of the microscopic field.

29. The method of claim 21 further comprising:

illuminating said sample to thereby generate a third plurality of interacted photons from a third set of multiple points, wherein

said third plurality of interacted photons is selected from the group consisting of;

photons absorbed by said sample, photons reflected by said sample, photons emitted by said sample, and combinations thereof, and wherein

said third set of multiple points corresponds to at least one point in said second set of multiple points wherein said hazardous agent was detected; and

assessing said third plurality of interacted photons to thereby determine at least one of;

the presence of a hazardous agent and the absence of a hazardous agent.

30. A device for assessing the occurrence of a hazardous agent in a sample, the device comprising:

a radiation source for irradiating said sample;

a detector for simultaneously detecting a first plurality of interacted photons from multiple discrete portions of said sample; and

a controller operably linked to said detector for restricting detection of said first plurality of interacted photons to the multiple discrete portions of said sample.

31. The device of claim 30 further comprising a tunable filter.

32. The device of claim 30 wherein said detector is selected from the group consisting of:

an infrared detector, a fluorescence detector, a UV/visible detector, and combinations thereof.