Methods for making and using SPR microarrays

• US 8,094,315 B2
• Filed: 12/08/2008
• Issued: 01/10/2012
• Est. Priority Date: 12/06/2005
• Status: Active Grant

• Current Assignee: Suzhou Puxin Life Science & Technology Limited
• Original Assignee: Plexera, LLC
• Inventors: Wolf, Nicholas Adam, Jin, Danliang, Barklund, Anna, Dinu, Raluca
• Primary Examiner(s): ZIMMER, MARC S
• Application Number:
  US12/330,384
• Publication Number:
  US 20090093067A1
• Time in Patent Office: 1,128 Days
• Field of Search: None
• US Class Current: 356/445
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/553 Metal or metal coated
• CPC Class Codes: Y10T 428/12493 Composite; i.e., plural, adjacent, spatially distinct metal components [e.g., layers, joint, etc.]
• CPC Class Codes: Y10T 428/12535 with additional, spatially distinct nonmetal component
• CPC Class Codes: Y10T 428/12556 Organic component
• CPC Class Codes: Y10T 428/1259 Oxide
• CPC Class Codes: Y10T 428/12597 Noncrystalline silica or noncrystalline plural-oxide component [e.g., glass, etc.]
• CPC Class Codes: Y10T 428/12861 Group VIII or IB metal-base component
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

An article, process, and method for surface plasmon resonance plates are described. A substrate is covered with a thin metal film onto which a second thin metal film is deposited. The surface of the second thin metal film is converted to the metal oxide which is used to covalently bond organosilanes to the surface. Reactive organosilanes containing terminal bonding groups are arranged in a plurality of spots that are surrounded by inert organosilanes. Biomolecule attachment to the binding group is detected or measured from surface plasmon signals from the first thin metal film.

Claims

1. A method of determining binding between a binding group and a biomolecule comprising:

a) providing an article including;

i) a substrate;

ii) a first metal film overlying the substrate;

iii) a second thin metal selected from the group consisting of Ti, Zr, Al, Cr, Hf, V, Ta, W and Pb, wherein the thin metal film includes a proximal surface and a distal surface, wherein the proximal surface is proximal to the first metal film and the distal surface is distal to the first metal film; and

iv) the binding group attached to the distal surface of the second thin metal film;

b) contacting the article with the biomolecule; and

c) measuring a surface plasmon resonance signal to determine the binding between the binding group and the biomolecule.

2. The method of claim 1, wherein the binding group is selected from the group consisting of DNA and RNA.

3. The method of claim 2, wherein the biomolecule is selected from the group consisting of DNA, RNA, and a protein.

4. The method of claim 1, wherein the binding group is selected from the group consisting of an antigen, an antibody, and a hapten.

5. The method of claim 1, wherein the first metal film is selected from the group consisting of Au, Ag, and Cu.

6. The method of claim 1, wherein the substrate is made from a material selected from the group consisting of glass and quartz.

7. An article for determining binding between a binding group and a biomolecule comprising:

i) a substrate;

ii) a first metal film overlying the substrate;

iii) a second thin metal film selected from the group consisting of Ti, Zr, Al, Cr, Hf, V, Ta, W and Pb, wherein the second thin metal film includes a proximal surface and a distal surface, wherein the proximal surface is proximal to the first metal film and the distal surface is distal to the first metal film; and

iv) the binding group attached to the distal surface of the second thin metal film, wherein contacting the article with the biomolecule and measuring a surface plasmon resonance signal determines binding between the binding group and the biomolecule.

8. The article of claim 7, wherein the first metal film is selected from the group consisting of Au, Ag, and Cu.

9. The article of claim 7, wherein the substrate is made from a material selected from the group consisting of glass and quartz.

10. The article of claim 7, wherein the binding group is selected from the group consisting of DNA and RNA.