Addressable chem/bio chip array

• US 8,097,134 B2
• Filed: 04/01/2005
• Issued: 01/17/2012
• Est. Priority Date: 04/01/2004
• Status: Expired due to Fees

• Current Assignee: Nanyang Technological University
• Original Assignee: Nanyang Technological University
• Inventors: Li, Changming
• Primary Examiner(s): Barton, Jeffrey T
• Assistant Examiner(s): Ball, J. Christopher
• Application Number:
  US11/097,945
• Publication Number:
  US 20050252777A1
• Time in Patent Office: 2,482 Days
• Field of Search: 204/600, 204/400, 204/403.01, 204/267, 205/775
• US Class Current: 204/403.01
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00704 integrated with the reactor apparatus
• CPC Class Codes: B01J 2219/00713 Electrochemical synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68
• CPC Class Codes: G01N 33/5438 : Electrodes

Abstract

Addressable bio/chem chips include a plurality of isolated test cells, each addressable by at least two electrodes for measuring electrical characteristics of probe/test molecule interactions. In one embodiment, electrodes are located within a channel to allow for four terminal measurement. In another embodiment, electrodes are arranged in rows and columns and interconnected with electrolyte pads used as test sites. In yet another embodiment, electrodes are arranged in cells, with each cell including a counter electrode surrounded encircled by working electrodes.

Claims

1. An addressable chip comprising:

a substrate;

a plurality of counter electrodes formed on said substrate;

a plurality of reference electrodes, each one substantially encircling a respective counter electrode one of said plurality of counter electrodes in the same plane as its respective counter electrodes;

a plurality of working electrodes, groups of said plurality of working electrodes encircling each of said plurality of reference electrodes and electrically isolated from said counter electrodes;

pairs of said counter/working electrodes for interconnection with a voltage source, to generate a potential for detection of chem/biological probe and target molecule interaction proximate a pair of counter/working electrodes.

2. The chip of claim 1, wherein said substrate comprises a planar surface is formed of hydrophobic material.

3. The chip of claim 2, wherein said planar surface of said substrate is coated with a hydrophobic layer.

4. The chip of claim 1, wherein said working and counter electrodes are formed of at least one of platinum, gold, metal, carbon, carbides, nitrides, and conductive polymers.

5. The chip of claim 1, further comprising at least one wall isolating fluids proximate each of said counter electrodes from other ones of said counter electrodes.

6. The chip of claim 1, further comprising at least one trench isolating fluids proximate each of said counter electrodes from other ones of said counter electrodes.

7. The chip of claim 1, wherein said substrate is formed of at least one of plastic, ceramic, glass, rubber, fabric, printed circuit board, and silicon.

8. The chip of claim 1, further comprising a plurality of interconnects allowing independent measurement of current flow between each one of said counter electrodes and one of said working electrodes about said each one of said counter electrodes.

9. The chip of claim 8, wherein one working electrode about each of said counter electrodes is interconnected with one working electrode of each of said groups of working electrodes.

10. The chip of claim 1, wherein each of said counter electrodes is generally rectangular, square or circular in shape.

11. The chip of claim 1, further comprising a plurality of electrolyte pads, each covering one of said counter electrodes and its surrounding group of working electrodes.

12. The chip of claim 11, wherein said electrolyte pads are insoluble.

13. The chip of claim 11, wherein said electrolyte pads are formed of an electrolyte selected from the group consisting of polyethylene oxide, polyvinyl(alcohol), polyvinyl acetate, polyacrylamide, poly(vinylpyridine), polyethyleneimine and their combinations.

14. A test apparatus comprising the chip of claim 1;

and a measuring device for measuring electrical characteristics at a selected pair of said counter/working electrodes, to analyse an analyte proximate said one of said plurality pair of counter/working electrodes.

15. The test apparatus of claim 14, further comprising at least one inductor interconnected between said measuring device and said pair of said counter/working electrodes.

16. The test apparatus of claim 14, wherein said measuring device measures the frequency response of said selected pair of said counter/working electrodes.

17. The test apparatus of claim 16, further comprising at least one electrolyte pad covering at least said selected pair of said counter/working electrodes.

18. The test apparatus of claim 17, wherein said electrolyte pad is formed of cross-linked hydrogel.

19. The test apparatus of claim 17, wherein said electrolyte pad is formed of polyacryamide.

20. The test apparatus of claim 14, wherein said measuring device measures variation in impedance of said selected pair of said counter/working electrodes near a resonant frequency of said selected pair of said counter/working electrodes.

21. The test apparatus of claim 14, wherein said measuring device comprises a variable frequency voltage source.