Roundworm coproantigen detection

• US 8,097,261 B2
• Filed: 06/11/2010
• Issued: 01/17/2012
• Est. Priority Date: 06/15/2007
• Status: Active Grant

• Current Assignee: IDEXX Laboratories Incorporated
• Original Assignee: IDEXX Laboratories Incorporated
• Inventors: Elsemore, David Allen, Flynn, Laurie A.
• Primary Examiner(s): Swartz, Rodney P.
• Application Number:
  US12/814,207
• Publication Number:
  US 20100248217A1
• Time in Patent Office: 585 Days
• Field of Search: 424/130.1, 424/141.1, 424/151.1, 424/184.1, 424/265.1, 435/7, 435/7.1, 435/287.1
• US Class Current: 424/265.1
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: G01N 2333/4353 from nematodes
• CPC Class Codes: G01N 2800/06 Gastro-intestinal diseases
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Abstract

A composition, device, kit and method for detecting the presence or absence of roundworm in a fecal sample. The composition, device, kit and method of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm.

Claims

1. A device for detecting the presence or absence of Toxocara roundworm antigen in a fecal preparation from a mammal, comprising a solid support, wherein one or more antibodies specific for one or more Toxocara roundworm antigens are immobilized on the solid support.

2. The device of claim 1 wherein the one or more antibodies specific for one or more Toxocara roundworm antigens are not specific for any coproantigen present in the fecal preparation selected from the group consisting of hookworm, whipworm, and heartworm.

3. The device of claim 1 wherein the one or more antibodies are specific for an extract of whole Toxocara roundworm, an extract of Toxocara roundworm intestine or an extract of Toxocara roundworm reproductive organ.

4. The device of claim 3 wherein the Toxocara roundworm is Toxocara canis.

5. The device of claim 1 wherein one or more of the one or more antibodies are specifically bound to one or more Toxocara roundworm coproantigens.

6. The device of claim 1 wherein one or more of the one or more antibodies are labeled.

7. The device of claim 1 wherein the device is an enzyme-linked immunosorbent assay device.

8. The device of claim 7 wherein the enzyme-linked immunosorbent assay device is a lateral flow immunoassay device.

9. The device of claim 1 wherein the mammal is a canine or a feline.

10. The device of claim 1 wherein the device further includes one or more reagents for the detection of one or more of the group consisting of:

one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, and one or more bacteria, said one or more reagents selected from the group consisting of one or more antibodies or one or more antigens recognized by antibodies specific for one or more non-roundworm parasites, one or more non-worm parasites, one or more viruses and one or more bacteria.

11. The device of claim 1, wherein the one or more Toxocara roundworm antigens is Toxocara canis antigen or Toxocara cati antigen.

22. A kit for detection of one or more Toxocara roundworm antigens in a sample of a mammal, the kit comprising the device of any one of claims 1-11 and 12-21, and one or more reagents sufficient for the detection of the one or more Toxocara roundworm antigens.

23. The kit of claim 22 wherein the one or more reagents are selected from the group consisting of one or more indicator reagents, one or more antibody labeling compounds, one or more enzyme substrates, one or more antibodies specific for one or more Toxocara roundworm antigens, one or more anti-species antibodies, one or more antigen capture reagents, one or more inhibitors, and one or more wash reagents.

24. The kit of claim 22 wherein the one or more Toxocara roundworm antigens are Toxocara roundworm coproantigens.

25. The device of claim 1 wherein the one or more Toxocara roundworm antigens are Toxocara roundworm coproantigens.

12. A device for determining the presence or absence of an intestinal Toxocara roundworm infection in a mammal, the device comprising a solid support, wherein one or more antibodies specific for one or more Toxocara roundworm coproantigens are immobilized on the solid support.

13. The device of claim 12 wherein the one or more antibodies specific for one or more Toxocara roundworm coproantigens are not specific for any coproantigen from the mammal selected from the group consisting of hookworm, whipworm, and heartworm.

14. The device of claim 12 wherein the one or more antibodies are specific for an extract of whole Toxocara roundworm, an extract of Toxocara roundworm intestine or an extract of Toxocara roundworm reproductive organ.

15. The device of claim 12 wherein the Toxocara roundworm is Toxocara canis.

16. The device of claim 12 wherein one or more of the one or more antibodies are specifically bound to one or more Toxocara roundworm coproantigens.

17. The device of claim 12 wherein one or more of the one or more antibodies are labeled.

18. The device of claim 12 wherein the device is an enzyme-linked immunosorbent assay device.

19. The device of claim 18 wherein the enzyme-linked immunosorbent assay device is a lateral flow immunoassay device.

20. The device of claim 12 wherein the mammal is a canine or a feline.

21. The device of claim 12, wherein the one or more Toxicara roundworm coproantigens are Toxocara canis or Toxocara cati coproantigens.