Freeze-dried platelets, method of making and method of use as a diagnostic agent
First Claim
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1. A freeze-dried platelet composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose made by the process of:
- obtaining fresh platelets;
incubating the platelets in DMSO;
isolating the platelets by centrifugation;
resuspending the platelets in a loading buffer which comprises trehalose and ethanol thereby forming a first mixture;
incubating the first mixture;
mixing cryoprecipitate or a fraction thereof and polysucrose with the first mixture, thereby forming a second mixture; and
lyophilizing the second mixture to form a freeze dried composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose.
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Abstract
The present invention provides freeze-dried platelets and compositions comprising freeze-dried platelets for use as a diagnostic and calibration reagent for blood coagulation function. The invention also provides methods of diagnosing or monitoring blood coagulation function, including diagnosing or monitoring blood coagulation diseases and disorders. Kits for performing the methods of the invention are also included.
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Citations
19 Claims
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1. A freeze-dried platelet composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose made by the process of:
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obtaining fresh platelets; incubating the platelets in DMSO; isolating the platelets by centrifugation; resuspending the platelets in a loading buffer which comprises trehalose and ethanol thereby forming a first mixture; incubating the first mixture; mixing cryoprecipitate or a fraction thereof and polysucrose with the first mixture, thereby forming a second mixture; and lyophilizing the second mixture to form a freeze dried composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method of making a freeze-dried platelet composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose comprising:
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obtaining fresh platelets; incubating the platelets in DMSO; isolating the platelets by centrifugation; resuspending the platelets in a loading buffer which comprises trehalose and ethanol thereby forming a first mixture; incubating the first mixture; mixing cryoprecipitate or a fraction thereof and polysucrose with the first mixture, thereby forming a second mixture; and lyophilizing the second mixture to form a freeze-dried composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose. - View Dependent Claims (18)
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19. A rehydrated platelet composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose made by the process of:
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obtaining fresh platelets; incubating the platelets in DMSO; isolating the platelets by centrifugation; resuspending the platelets in a loading buffer which comprises trehalose and ethanol thereby forming a first mixture; incubating the first mixture; mixing cryoprecipitate or a fraction thereof and polysucrose with the first mixture, thereby forming a second mixture; lyophilizing the second mixture to form a freeze-dried composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose; and rehydrating the freeze-dried composition.
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Specification