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Freeze-dried platelets, method of making and method of use as a diagnostic agent

  • US 8,097,403 B2
  • Filed: 12/14/2007
  • Issued: 01/17/2012
  • Est. Priority Date: 12/14/2006
  • Status: Active Grant
First Claim
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1. A freeze-dried platelet composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose made by the process of:

  • obtaining fresh platelets;

    incubating the platelets in DMSO;

    isolating the platelets by centrifugation;

    resuspending the platelets in a loading buffer which comprises trehalose and ethanol thereby forming a first mixture;

    incubating the first mixture;

    mixing cryoprecipitate or a fraction thereof and polysucrose with the first mixture, thereby forming a second mixture; and

    lyophilizing the second mixture to form a freeze dried composition comprising platelets, cryoprecipitate or a fraction thereof, polysucrose and trehalose.

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