Luminescent indicator dye and optical sensor

• US 8,097,725 B2
• Filed: 12/03/2004
• Issued: 01/17/2012
• Est. Priority Date: 12/03/2004
• Status: Active Grant

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: He, Huarui, Mortellaro, Mark A., Young, Susanne T.
• Primary Examiner(s): DESAI, RITA J
• Application Number:
  US11/003,163
• Publication Number:
  US 20060121623A1
• Time in Patent Office: 2,601 Days
• Field of Search: 546/79, 734/35
• US Class Current: 546/79
• CPC Class Codes: C07D 221/14 Aza-phenalenes, e.g. 1,8-naphthalimide
• CPC Class Codes: C07D 311/82 Xanthenes
• CPC Class Codes: C07F 5/022 without C-boron linkages
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

The present invention relates to a chemical compound that has applications as a luminescent indicator dye, and to an optical sensor, typically employed for determination of near-neutral pH values of aqueous samples. The optical sensor has particular application in the pH determination of body liquids such as, for example, blood, plasma and serum.

Claims

1. A compound having the general Formula I

2. The compound of claim 1, wherein R₂ and/or R₄ represent chlorine or fluorine, and R₁ and R₃ represent hydrogen.

4. An optical sensor for determining the pH of aqueous media comprising a luminescent dye and a matrix, wherein

said luminescent dye is a compound according to claim 1, and

said luminescent dye is immobilized to said matrix.

5. An optical sensor for determining the pH of aqueous media comprising a luminescent dye and a matrix, wherein

said luminescent dye is a compound according to claim 2, and

said luminescent dye is immobilized to said matrix.

7. A method of determining the pH of aqueous media comprising contacting an optical sensor according to claim 4 with said aqueous media.

8. The method of claim 7, wherein said aqueous media is selected from blood, plasma or serum.

9. A method of determining the pH of aqueous media comprising contacting an optical sensor according to claim 5 with said aqueous media.

10. The method of claim 9, wherein said aqueous media is selected from blood, plasma or serum.

3. A compound of the formula

6. An optical sensor for determining the pH of aqueous media comprising a luminescent dye and a matrix, wherein

said luminescent dye is a compound according to claim 3, and

said luminescent dye is immobilized to said matrix.

11. A method of determining the pH of aqueous media comprising contacting an optical sensor according to claim 6 with said aqueous media.

12. The method of claim 11, wherein said aqueous media is selected from blood, plasma or serum.