Label-free high-throughput optical technique for detecting biomolecular interactions
First Claim
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1. A method of detecting binding of one or more specific binding substances to their respective binding partners comprising:
- (a) illuminating a biosensor with light and detecting a first peak wavelength value (PWV), wherein the biosensor comprises;
(i) a two-dimensional grating;
(ii) a substrate that supports the two-dimensional grating;
wherein the refractive index of the two-dimensional grating is greater than the refractive index of the substrate; and
(iii) one or more specific binding substances immobilized on the surface of the two-dimensional grating opposite of the substrate;
wherein the one or more specific binding substances do not comprise detection labels, wherein when the biosensor is illuminated a resonant grating effect is produced on a reflected radiation spectrum, and wherein the depth and cross-sectional period of the two-dimensional grating are less than the wavelength of the resonant grating effect;
(b) applying one or more binding partners to the biosensor, wherein the binding partners do not comprise detection labels; and
(c) illuminating the biosensor with light and detecting a second PWV;
wherein, if the one or more specific binding substances have bound to their respective binding partners, then the second PWV is shifted as compared to the first PWV.
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Abstract
Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.
217 Citations
14 Claims
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1. A method of detecting binding of one or more specific binding substances to their respective binding partners comprising:
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(a) illuminating a biosensor with light and detecting a first peak wavelength value (PWV), wherein the biosensor comprises; (i) a two-dimensional grating; (ii) a substrate that supports the two-dimensional grating;
wherein the refractive index of the two-dimensional grating is greater than the refractive index of the substrate; and(iii) one or more specific binding substances immobilized on the surface of the two-dimensional grating opposite of the substrate;
wherein the one or more specific binding substances do not comprise detection labels, wherein when the biosensor is illuminated a resonant grating effect is produced on a reflected radiation spectrum, and wherein the depth and cross-sectional period of the two-dimensional grating are less than the wavelength of the resonant grating effect;(b) applying one or more binding partners to the biosensor, wherein the binding partners do not comprise detection labels; and (c) illuminating the biosensor with light and detecting a second PWV; wherein, if the one or more specific binding substances have bound to their respective binding partners, then the second PWV is shifted as compared to the first PWV. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification