Method and system for microfluidic device and imaging thereof

• US 8,105,550 B2
• Filed: 12/22/2009
• Issued: 01/31/2012
• Est. Priority Date: 05/20/2003
• Status: Expired due to Fees

• Current Assignee: Fluidigm Corporation
• Original Assignee: Fluidigm Corporation
• Inventors: Quan, Emerson Chueng, Taylor, Colin Jon, Lee, Michael, Cesar, Christopher G., Harris, Greg, Sun, Gang
• Primary Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US12/645,396
• Publication Number:
  US 20100166608A1
• Time in Patent Office: 770 Days
• Field of Search: 422/500, 422/50, 422/68.1, 436/43, 436/63
• US Class Current: 422/500
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: G01N 33/00 Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

Claims

1. A microfluidic system, the system comprising:

a rigid substrate material, the rigid substrate material having a surface region, the surface region being capable of acting as a handle substrate;

a deformable fluid layer coupled to the surface region;

one or more well regions formed in a first portion of the deformable fluid layer, the one or more well regions being capable of holding a fluid therein;

one or more channel regions formed in a second portion of the deformable fluid layer, the one or more channel regions being coupled to one or more of the well regions;

an active region formed in the deformable fluid layer, the active region including the one or more well regions;

a non-active region formed in the deformable fluid layer, the non-active region being formed outside of the first portion and the second portion;

a first fiducial marking formed within the non-active region and disposed in a spatial manner associated with at least one of the channel regions;

a second fiducial marking formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions;

a control layer coupled to the fluid layer, the control layer including one or more control regions; and

a third fiducial marking formed within the control layer.

2. The substrate of claim 1 wherein the second fiducial marking is a company logo and is larger than either the first fiducial marking or the second fiducial marking.

3. The substrate of claim 1 wherein the first fiducial marking comprises a first channel fiducial marking and a second channel fiducial marking, the first channel fiducial marking being spatially disposed from the second channel fiducial marking.

4. The substrate of claim 1 wherein the third fiducial marking comprises a first control fiducial marking and a second control fiducial marking, the first control fiducial marking being spatially disposed from the second control fiducial marking.

5. The substrate of claim 1 wherein the first fiducial marking, the second fiducial marking, and the third fiducial marking, being spatially disposed from each other along the surface region.

6. The substrate of claim 1 wherein the first fiducial marking is characterized by a spatial dimension of greater than 100 μ

m and less than 250 μ

m.

7. The substrate of claim 1 wherein the first fiducial marking is characterized by a depth of at least 10 μ

m within a thickness of the non-active region.

8. The substrate of claim 1 wherein the second fiducial marking is characterized by a depth of at least 10 μ

m within a thickness of the non-active region.

9. The substrate of claim 1 wherein the third fiducial marking is characterized by a depth of at least 10 μ

m within a thickness of the control layer.

10. A microfluidic system, the system comprising:

a substrate comprising a surface region;

a deformable layer coupled to the surface of the substrate, the deformable layer being made of at least a thickness of first material;

a control layer coupled to the deformable layer to form a sandwich structure including at least the substrate, the deformable layer and the control layer, the control layer being made of at least a thickness of second material;

at least one fiducial marking provided within either the control layer or the deformable layer or the substrate, the fiducial marking being characterized by a visual pattern provided in a volume surrounded wholly or partially by at least the substrate, the first material, or the second material; and

a fluid disposed within the open volume of the one fiducial marking, the fluid being characterized by a refractive index.

11. The system of claim 10 wherein the refractive index is associated with air.

12. The system of claim 10 wherein the fluid comprises an air mixture.

13. The system of claim 10 wherein the fluid comprises air.

14. The system of claim 10 wherein the open volume is characterized by a recessed region.

15. The system of claim 10 wherein the refractive index is substantially different from a surrounding material, the surrounding material being either the first material or the second material or the substrate.

16. The system of claim 10 wherein the fiducial marking is characterized by edges to form the pattern.

17. The system of claim 16 wherein the edges comprises 90 degree corners.

18. The system of claim 10 wherein the fluid is non-reactive.

19. The system of claim 10 wherein the substrate is selected from silicon, quartz, glass, or rigid plastic.