Optical sensor array system for parallel processing of chemical and biochemical information

• US 8,105,552 B2
• Filed: 03/24/2010
• Issued: 01/31/2012
• Est. Priority Date: 10/26/2005
• Status: Active Grant

• Current Assignee: BL Technologies Inc. (Ashland Global Holdings, Inc.)
• Original Assignee: General Electric Company
• Inventors: Xiao, Caibin, Potyrailo, Radislav A., Morris, William G., Boyette, Scott M., Yu, LiJie, Cecconie, Theodore J., Leach, Andrew M., Shrikhande, Prashant V.
• Primary Examiner(s): Jarrett, Lore
• Application Number:
  US12/730,550
• Publication Number:
  US 20100178208A1
• Time in Patent Office: 678 Days
• Field of Search: 422/502, 702/22, 702/147
• US Class Current: 422/502
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/088 by specific surface properties
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: Y10T 137/7036 Jacketed

Abstract

Total analysis systems and methods for simultaneously monitoring a suite of biological and/or chemical species in water and/or other process systems are disclosed. The system provides a sample-volume controlled sensor array comprising a fluid delivery device and a plurality of optical sensor elements for determining the presence and total concentrations of multiple analytes in the process system simultaneously. Image identification algorithms are provided for identifying the analytes based on image intensity, color pattern, positional arrangement, and the like. The methods incorporate multivariate optimization algorithms to analyze multiple sensor responses. This produces analytical results that are typically difficult to obtain without full system or variable compensation. The improved array response may then be utilized to measure, monitor, and control the concentration of analytes in the chemical or biological sample or water system.

Claims

1. A system for simultaneously measuring a plurality of analyte concentrations in a chemical or biological sample, comprising:

(a) a sensor array comprising a plurality of sensor elements, each said element responding to at least one of said plurality of analytes;

(b) at least one light source for directing light onto said sensor elements;

(c) a sample delivery device for delivering said sample to said sensor elements, wherein said sample delivery device comprises a sample entry port, a plurality of reservoirs, and a plurality of fluidic channels, said channels being connected between said reservoirs and said entry port, and a cover layer, wherein the cover layer has a hydrophobic outer surface, a hydrophilic lower surface, and a plurality of vent holes formed therein configured to be aligned with said reservoirs, wherein when sample is introduced into the system through the sample entry port, said sample is driven along the channels by the hydrophilic surface of the cover layer and into said reservoirs in order to create a chemical reaction between at least one analyte and said sensor elements, wherein air escapes through the vent holes as the reservoirs fill with sample and the hydrophobic outer surface prevents sample from flowing through the vent holes, thereby allowing a hydrostatic balance to be reached such that a fixed volume of sample is delivered to each reservoir and sensor element without requiring an external force other than gravity to move the sample through the sample delivery device;

(d) a detector for detecting a spectral profile and positional arrangement of light reflected from said sensor array, said detector adapted to convert said detected spectral profile and said positional arrangement into a digital record;

(e) an image identification algorithm for identifying said at least one analyte based on an image intensity, color pattern, positional arrangement, or combinations thereof; and

(f) a software-based optimization algorithm incorporating sensor responses from said sensor array to produce an analysis result with full system and variable compensation.

2. The system of claim 1, wherein said detector means comprises a digital or analog imaging device.

3. The system of claim 1, wherein said sensor array comprises multiple sensor elements of different chemistries for each said analyte.

4. The system of claim 1, wherein said sensor array comprises two or more films, each responsive to different or combinations of said analytes.

5. The system of claim 1, wherein said sensor array further comprises at least one internal reference standard incorporated into said sensor elements or positioned in proximity to said sensor elements.

6. The system of claim 1, wherein said delivery means comprises a sample entry port, a plurality of reservoirs, and a plurality of fluidic channels, said channels being connected between said reservoirs and said entry port.

7. The system of claim 6, wherein said channels and reservoirs are configured in a series or parallel-series branched-type structure.

8. The system of claim 7, further comprising a waste reservoir and a delay channel.

9. The system of claim 6, further comprising a top layer, a middle layer and a bottom layer, said middle layer having a plurality of grooves leading to a plurality of cut-outs, said bottom layer having a matching set of cut-outs, said channels and said reservoirs being formed from said grooves and said cut-outs, respectively, when said bottom layer is bonded to said middle layer, said top layer having a plurality of vent holes located above said reservoirs, said top layer having a sample entry port for introducing said sample into said channels.

10. The system of claim 9, further comprising a substrate with a plurality of sensor elements.

11. The system of claim 1 further comprising a base plate upon which the plurality of sensor elements are mounted, wherein the sample delivery means comprises the cover layer and a fluidic distribution layer, and wherein the fluidic distribution layer has a plurality of grooves and cutouts that form the channels and reservoirs.

12. The system of claim 11 further comprising a double-sided adhesive film with through holes cut to match said reservoirs in said fluidic distribution layer and binding said sensor element carrying base plate with said fluidic distribution layer.

13. A sampling system for simultaneously measuring a plurality of analyte concentrations in a chemical or biological sample, comprising:

a sensor array comprising a plurality of sensor elements, each said element responding to at least one of said plurality of analytes; and

a sample delivery device for delivering said sample to said sensor elements, wherein said sample delivery device comprises a sample entry port, a plurality of reservoirs, and a plurality of fluidic channels, said channels being connected between said reservoirs and said entry port, and a cover layer, wherein the cover layer has a hydrophobic outer surface, a lower surface, the entire lower surface being hydrophilic, and a plurality of vent holes formed therein configured to be aligned with said reservoirs, wherein when sample is introduced into the system through the sample entry port, said sample is driven along the channels by the hydrophilic surface of the cover layer and into said reservoirs in order to create a chemical reaction between at least one analyte and said sensor elements, wherein air escapes through the vent holes as the reservoirs fill with sample and the hydrophobic outer surface prevents sample from flowing through the vent holes, thereby allowing a hydrostatic balance to be reached such that a fixed volume of sample is delivered to each reservoir and sensor element without requiring an external force other than gravity to move the sample through the sample delivery device.