Method for pairwise sequencing of target polynucleotides
First Claim
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1. A method for pairwise sequencing of first and second regions of target polynucleotides, wherein each target polynucleotide comprises a first and second strand, the method comprising:
- (a) providing a solid support comprising immobilized first strands of the target polynucleotides;
(b) sequencing the first region of the first strands of the target polynucleotides;
(c) copying the first strands of the target polynucleotides thereby generating the second strands of the target polynucleotides; and
(d) sequencing the second region of the second strands of the target polynucleotides, wherein determining the sequence of the first and second regions of the target polynucleotides achieves pairwise sequencing of the first and second regions of the target polynucleotides.
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Abstract
The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
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Citations
20 Claims
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1. A method for pairwise sequencing of first and second regions of target polynucleotides, wherein each target polynucleotide comprises a first and second strand, the method comprising:
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(a) providing a solid support comprising immobilized first strands of the target polynucleotides; (b) sequencing the first region of the first strands of the target polynucleotides; (c) copying the first strands of the target polynucleotides thereby generating the second strands of the target polynucleotides; and (d) sequencing the second region of the second strands of the target polynucleotides, wherein determining the sequence of the first and second regions of the target polynucleotides achieves pairwise sequencing of the first and second regions of the target polynucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification