Biosensor article and methods thereof

• US 8,105,822 B2
• Filed: 11/18/2008
• Issued: 01/31/2012
• Est. Priority Date: 10/10/2007
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Goodrich, Terry T.
• Primary Examiner(s): Prouty, Rebecca
• Assistant Examiner(s): Martin, Paul
• Application Number:
  US12/273,147
• Publication Number:
  US 20100285453A1
• Time in Patent Office: 1,169 Days
• Field of Search: None
• US Class Current: 435/287.9
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: G01N 33/551 the carrier being inorganic

Abstract

A biosensor or cell culture article including: a substrate having a bio-compatible layer. The bio-compatible layer can be obtained from surface oxidation of a substrate coating comprising the reaction product of a suitable oxidizable polymer and modifier compound, such as a triamine, attached to the oxidizable polymer. Methods for making the biosensor or cell culture article and methods for performing an assay of a ligand with the biosensor article are also disclosed.

Claims

1. A biosensor article comprising:

a substrate;

a first-tie layer attached to at least the substrate;

a bio-compatible layer directly or indirectly attached to the substrate; and

a second tie-layer attached to the bio-compatible layer, the bio-compatible layer comprises the oxidation product of the second tie-layer and a polymer, the polymer being selected from a poly(styrene-co-maleic anhydride), poly(styrene-maleic acid), or a combination thereof, and the substrate surface is comprised of a metal oxide, SiO₂, a mixed metal oxide, cyclic olefin copolymer, or a combination thereof.

2. The article of claim 1, wherein the second tie-layer comprises a polyether triamine.

3. The article of claim 2, wherein the molecular weight of the polyether triamine is from about 200 to about 1,000 relative to hydrogen.

4. The article of claim 2, wherein the molecular weight of the polyether triamine is from about 300 to about 500 relative to hydrogen.

5. The article of claim 1, wherein the surface of the substrate comprises a metal oxide or a mixed metal oxide.

6. The article of claim 1, wherein the bio-compatible layer is directly attached to the substrate surface.

7. The article of claim 1, wherein the surface of the substrate comprises a cyclic olefin copolymer.

8. The article of claim 1, wherein the second tie-layer comprises a polyether diamine.

21. The article of claim 1 wherein biocompatible layer comprises a nanoparticle dispersion.

9. A method for preparing a biosensor article comprising:

providing a biosensor comprising;

a substrate comprised of a metal oxide, SiO₂, a mixed metal oxide, cyclic olefin copolymer, or a combination thereof;

a first-tie layer attached to at least the substrate;

a polymer directly or indirectly attached to the substrate, the polymer being selected from a poly(styrene-co-maleic anhydride), poly(styrene-maleic acid), or a combination thereof; and

a second tie-layer attached to the polymer,

the polymer being attached to the surface of the first-tie layer, and

oxidizing the surface of the biosensor to form a bio-compatible surface.

10. The method of claim 9, wherein oxidizing comprises contacting the surface with UV-ozone, a plasma, or both to produce the bio-compatible surface.

11. The method of claim 10, further comprising associating a bio-material with resulting bio-compatible surface.

12. The method of claim 9, wherein the second tie-layer comprises a polyether triamine having a molecular weight of from about 200 to about 1,000 relative to hydrogen.

13. The method of claim 9, wherein the polymer oxidation product comprises a polymer comprising mer units of at least one of styrene, maleic acid, acrylic acid, acrylamide, acrolein, methylvinylketone, or combinations thereof, and the second tie-layer comprises a polyether triamine having a molecular weight of from about 200 to about 1,000 relative to hydrogen.

20. A biosensor article or cell culture article made by the method of claim 9.

14. A method for performing an assay of a ligand, the method comprising:

contacting the ligand with a biosensor article having a bio-material associated with the bio-compatible layer, and

if the ligand binds to the bio-material, then;

detecting the ligand-induced response of the bio-material with the biosensor, the biosensor comprising;

a substrate;

a first-tie layer attached to at least the substrate;

a bio-compatible layer directly or indirectly attached to the substrate; and

a second tie-layer attached to the bio-compatible layer, the bio-compatible layer comprises the oxidation product of the second tie-layer and a polymer, the polymer being selected from a poly(styrene-co-maleic anhydride), poly(styrene-maleic acid), or a combination thereof, and the substrate surface is comprised of a metal oxide, SiO₂, a mixed metal oxide, cyclic olefin copolymer, or a combination thereof.

15. The method of claim 14, wherein the ligand comprises, a therapeutic candidate compound, a prophylactic candidate, a prophylactic agent, a peptide, a protein, a small molecule drug candidate having a molecular weight of less than about 500 Daltons, a biologic drug candidate, a small molecule-biologic conjugate, a pathogen, or combinations thereof, and the bio-material comprises a natural or synthetic oligonucleotide, a natural or synthetic nucleotide/nucleoside, a nucleic acid (DNA or RNA), a natural peptide, a natural or synthetic peptide optionally comprising one or more modified or blocked amino acids, an antibody, a hapten, a biological ligand, a protein membrane, a lipid membrane, a protein, a small molecule having a molecular weight of less than about 500 Daltons, a cell, or a combination thereof, or a conjugate thereof.

16. The method of claim 14, wherein the biosensor comprises at least one of a surface plasmon resonance biosensor, a waveguide resonant grating biosensor, an impedance biosensor, a mass spectrometry biosensor, or a combination thereof.

17. The method of claim 14, wherein the biomaterial comprises a live-cell.

18. A cell culture article comprising:

a substrate;

a first-tie layer attached to at least the substrate;

a bio-compatible layer directly or indirectly attached to the substrate; and

a second tie-layer attached to the bio-compatible layer, the bio-compatible layer comprises the oxidation product of the second tie-layer and a polymer, the polymer being selected from a poly(styrene-co-maleic anhydride), poly(styrene-maleic acid), or a combination thereof, and the substrate surface is comprised of a metal oxide, SiO₂, a mixed metal oxide, cyclic olefin copolymer, or a combination thereof.

19. The article of claim 18, wherein the second tie-layer comprises a polyether triamine, a polyether diamine, or a combination thereof.

22. The article of claim 18 wherein biocompatible layer comprises a dispersion.