Integrated chip carriers with thermocycler interfaces and methods of using the same
First Claim
1. A method of operating a carrier for holding a microfluidic device comprising:
- (a) providing the carrier for holding the microfluidic device, wherein the carrier comprises;
a non-elastomeric substrate comprising a plurality of wells, wherein each of the plurality of wells has a well opening with a center point and the plurality of wells is spatially arranged such that the center point to center point spacing is about 4.5 mm;
a plurality of channels within the substrate wherein each of the plurality of wells is in fluid communication with at least one of the plurality of channels; and
a receiving portion on the non-elastomeric substrate for receiving the microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells, wherein the microfluidic device comprises an elastomeric material;
(b) providing the microfluidic device, the microfluidic device comprising a chamber wherein the chamber is coupled to a well through a channel in the substrate;
(c) providing a platen with an interface plate comprising one or more ports;
(d) introducing a reagent into at least one of the wells;
(e) urging the platen against an upper face of the substrate to form a pressure cavity;
(f) applying a pressure to at least one of the one or more ports; and
(g) driving fluid from the well containing the reagent into the chamber in the microfluidic device.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods and systems are provided for conducting a reaction at a selected temperature or range of temperatures over time. An array device is provided. The array device contains separate reaction chambers and is formed as an elastomeric block from multiple layers. At least one layer has at least one recess that recess has at least one deflectable membrane integral to the layer with the recess. The array device has a thermal transfer device proximal to at least one of the reaction chambers. The thermal transfer device is formed to contact a thermal control source. Reagents for carrying out a desired reaction are introduced into the array device. The array device is contacted with a thermal control device such that the thermal control device is in thermal communication with the thermal control source so that a temperature of the reaction in at least one of the reaction chamber is changed as a result of a change in temperature of the thermal control source.
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Citations
7 Claims
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1. A method of operating a carrier for holding a microfluidic device comprising:
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(a) providing the carrier for holding the microfluidic device, wherein the carrier comprises; a non-elastomeric substrate comprising a plurality of wells, wherein each of the plurality of wells has a well opening with a center point and the plurality of wells is spatially arranged such that the center point to center point spacing is about 4.5 mm; a plurality of channels within the substrate wherein each of the plurality of wells is in fluid communication with at least one of the plurality of channels; and a receiving portion on the non-elastomeric substrate for receiving the microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells, wherein the microfluidic device comprises an elastomeric material; (b) providing the microfluidic device, the microfluidic device comprising a chamber wherein the chamber is coupled to a well through a channel in the substrate; (c) providing a platen with an interface plate comprising one or more ports; (d) introducing a reagent into at least one of the wells; (e) urging the platen against an upper face of the substrate to form a pressure cavity; (f) applying a pressure to at least one of the one or more ports; and (g) driving fluid from the well containing the reagent into the chamber in the microfluidic device. - View Dependent Claims (2)
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3. A method of operating a carrier for holding a microfluidic device comprising:
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(a) providing the carrier for holding the microfluidic device, wherein the carrier comprises; a non-elastomeric substrate comprising a plurality of wells, wherein each of the plurality of wells has a well opening with a center point and the plurality of wells is spatially arranged such that the center point to center point spacing is about 4.5 mm; a plurality of channels within the substrate wherein each of the plurality of wells is in fluid communication with at least one of the plurality of channels; and a receiving portion on the non-elastomeric substrate for receiving the microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells, wherein the microfluidic device comprises an elastomeric material; (b) providing the microfluidic device comprising a chamber and a valve wherein the chamber is coupled to a well through a channel in the substrate and wherein the valve is coupled to an accumulator through a channel in the substrate; (c) providing a platen with an interface plate comprising more than one port, (d) introducing a reagent into at least one of the wells, (e) urging the platen against an upper face of the substrate to form a pressure cavity, (f) applying a pressure to a first port, (g) driving fluid from the well containing the reagent into the chamber in the microfluidic device, and (h) applying a pressure a second port to apply a pressure to the accumulator. - View Dependent Claims (4)
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5. A method of operating a carrier for holding a microfluidic device comprising:
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(a) providing the carrier for holding the microfluidic device, wherein the carrier comprises; a non-elastomeric substrate comprising a plurality of wells, wherein each of the plurality of wells has a well opening with a center point and the plurality of wells is spatially arranged such that the center point to center point spacing is about 4.5 mm; a plurality of channels within the substrate wherein each of the plurality of wells is in fluid communication with at least one of the plurality of channels; and a receiving portion on the non-elastomeric substrate for receiving the microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells, wherein the microfluidic device comprises an elastomeric material; (b) providing the microfluidic device comprising a chamber and a valve wherein the chamber is coupled to a well through a channel in the substrate and wherein the valve is coupled to an accumulator through a channel in the substrate; (c) providing a platen with an interface plate comprising one or more ports, (d) introducing a reagent into at least one of the wells, (e) urging the platen against an upper face of the substrate to form a pressure cavity, (f) applying a pressure to at least one of the one or more ports, (g) driving fluid from the well containing the reagent into the chamber in the microfluidic device, and (h) releasing a pressure in the accumulator.
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6. A method of operating a carrier for holding a microfluidic device comprising:
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(a) providing the carrier for holding the microfluidic device, wherein the carrier comprises; a non-elastomeric substrate comprising a plurality of wells, wherein each of the plurality of wells has a well opening with a center point and the plurality of wells is spatially arranged such that the center point to center point spacing is about 4.5 mm; a plurality of channels within the substrate wherein each of the plurality of wells is in fluid communication with at least one of the plurality of channels; a pressure accumulator; and a receiving portion on the non-elastomeric substrate for receiving the microfluidic device and placing the microfluidic device in fluid communication with the plurality of wells, wherein the receiving portion comprises a thermal control device and the microfluidic device comprises an elastomeric material; (b) providing the microfluidic device, the microfluidic device comprising a chamber and a valve wherein the chamber is coupled to a well through a channel in the substrate and wherein the valve is coupled to the accumulator through a channel in the substrate, (c) providing a platen with an interface plate comprising more than one port, (d) providing a thermal control source, (e) introducing a reagent into at least one of the wells, (f) urging the platen against an upper face of the substrate to form a pressure cavity, (g) applying a pressure to a first port, (h) driving fluid from the well containing the reagent into a chamber in the microfluidic device, (i) applying a pressure to a second port to apply a pressure in an accumulator, and (j) placing the thermal control device in thermal communication with the thermal control source so that a temperature in the chamber is changed as a result of a change in temperature of the thermal control source. - View Dependent Claims (7)
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Specification