Stem cells of the islets of langerhans and their use in treating diabetes mellitus
First Claim
1. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;
wherein said nestin-positive stem cells of said population are not neural stem cells,wherein said population is at least 30% pure; and
wherein said nestin-positive stem cells grow in RPMI-1640 .
2 Assignments
0 Petitions
Accused Products
Abstract
Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin and ABCG2 have been identified as molecular markers for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin and/or ABCG2-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.
2 Citations
17 Claims
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1. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells of said population are not neural stem cells, wherein said population is at least 30% pure; and wherein said nestin-positive stem cells grow in RPMI-1640 . - View Dependent Claims (2, 3, 4, 5, 6, 7)
- 6 and integrin subunit β
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8. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells are not neural stem cells; wherein said stem cell population is at least 40% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
-
9. A population of nestin-positive human pancreatic stem cells that wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;
wherein said nestin-positive stem cells are not neural stem cells;wherein said population is at least 50% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
-
10. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2;
-
wherein said nestin-positive stem cells are not neural stem cells; wherein said population is at least 60% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
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11. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells are not neural stem cells; wherein said population is at least 70% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
-
12. A population of nestin-positive human pancreatic stem cells that wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells are not neural stem cells; wherein said population is at least 80% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
-
13. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R or ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells are not neural stem cells; wherein said population is at least 85% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
-
14. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R or ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells are not neural stem cells; wherein said population is at least 90% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
-
15. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells are not neural stem cells; wherein said population is at least 95% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
-
16. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1 R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells are not neural stem cells; wherein said population is at least 98% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
-
17. A population of nestin-positive human pancreatic stem cells wherein said nestin-positive stem cells are also positive for at least one of GLP-1R, ABCG2, integrin subunit α
- 6 and integrin subunit β
1;wherein said nestin-positive stem cells are not neural stem cells; wherein said population is at least 99% pure; and wherein said nestin-positive stem cells grow in RPMI-1640.
- 6 and integrin subunit β
Specification